|
|||||
|
|
Application of Probe Electrospray Ionization Mass Spectrometry (PESI-MS) to Clinical Diagnosis: Solvent Effect on Lipid Analysis |
|
| Authors: | Mridul Kanti Mandal Kentaro Yoshimura Lee Chuin Chen Zhan Yu Tadao Nakazawa Ryohei Katoh Hideki Fujii Sen Takeda Hiroshi Nonami Kenzo Hiraoka |
| Institution: | 1. Clean Energy Research Center, University of Yamanashi, Kofu, Yamanashi, Japan 2. Department of Anatomy and Cell Biology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi, Japan 3. Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi, Japan 4. College of Chemistry and Biology, Shenyang Normal University, Shenyang, China 5. Department of Human Pathology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi, Japan 6. Department of Surgery, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi, Japan 7. Plant Biophysics/Biochemistry Research Laboratory, Faculty of Agriculture, Ehime University, Matsuyama, Japan |
| Abstract: | We have examined several combinations of solvents with the aim of optimizing the ionization conditions for molecular diagnosis of malignant tumours by PESI-MS. Although the best conditions may depend on the actual species in the sample, the optimal conditions for renal cell carcinoma (RCC) were achieved by using alcohols. PESI-MS successfully delineated the differential expression of phospholipids (PCs) and triacylglycerols (TAGs) in noncancerous and RCC tissues by using these solvent systems. This study paves the way for the application of PESI-MS in medical samples. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |