| 脂肪酶的固定化及其在有机酶促反应中稳定性研究 |
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| 引用本文： | 辛嘉英,李树本.脂肪酶的固定化及其在有机酶促反应中稳定性研究[J].分子催化,1999,13(2):103-108. |
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| 作者姓名： | 辛嘉英 李树本 |
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| 作者单位： | 中国科学院兰州化学物理研究所羰基合成与选择氧化国家重点实验室 |
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| 基金项目： | 中国科学院重点项目,中国科学院西部之光项目 |
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| 摘 要： | 利用吸附法，将圆柱状假丝酵母脂肪酶固定于４种疏水性不同的载体上，固定化酶的活性及稳定性随载休疏水性的增大而增大。用ＹＧＷ－Ｃ６Ｈ５作为载体，在有机溶剂－水双液相体系中催化萘普生甲酯的不对称水解，反应１２０ｈ转化率为２４．７２％，产品的对映体过量值为９４．８２％。
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| 关 键 词： | 脂肪酶 固定化酶 萘普生甲酯 不对称水解 拆分 |
Studies on the Immobilization and Stability of Lipase in Organic Media |
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| XIN Jiaying,LI Shuben XU YiWANG Lailai Shen Runnan YU Chili.Studies on the Immobilization and Stability of Lipase in Organic Media[J].Journal of Molecular Catalysis (China),1999,13(2):103-108. |
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| Authors: | XIN Jiaying LI Shuben XU YiWANG Lailai Shen Runnan YU Chili |
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| Institution: | State Key Laboratory for Oxo Synthesis & Selective Oxidation Lanzhou Institute of Chemical Physics The Chinese Academy of SciencesLanzhou 730000 |
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| Abstract: | In this paper the properties of Candida rugosa lipase immobilized on four kinds of support by an absorption method in organic\|aqueous biphase system have been studied. It was found that the immobilized lipase in organic media remained a very high activity, which was highly dependent on the nature of support material. The immobilized lipase activity in organic\|aqueous biphase media decreased with increasing hydrophilicity of the support materials. With YWG\|C6H5 as the support for lipase immobilization, 94.82% enantiomeric excess of naproxen could be obtain with a 24.72% conversion of naproxen methyl ester. The lactose addition showed an improvement in immobilized enzyme stability, while the half\|life period of immobilized enzyme was more than 1000 h. |
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| Keywords: | Immobilized lipase Hydrophobicity of support Naproxen Asymmetric hydrolysis |
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