Anti-epidermal growth factor receptor (anti-EGFR) antibody conjugated fluorescent nanoparticles probe for breast cancer imaging |
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Authors: | Xu Hun Zhujun Zhang |
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Institution: | aCollege of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China;bKey Laboratory of Eco-chemical Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China;cDepartment of Chemistry, School of Chemistry and Materials Science, Shaanxi Normal University, Xi’an 710062, China |
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Abstract: | Fluorescent nanoparticles (FNs) with unique optical properties may be useful as biosensors in living cancer cell imaging and cancer targeting. In this study, anti-EGFR antibody conjugated fluorescent nanoparticles (FNs) (anti-EGFR antibody conjugated FNs) probe was used to detect breast cancer cells. FNs with excellent character such as non-toxicity and photostability were first synthesized with a simple, cost-effective and environmentally friendly modified Stőber synthesis method, and then successfully modified with anti-EGFR antibody. This kind of fluorescence probe based on the anti-EGFR antibody conjugated FNs has been used to detect breast cancer cells with fluorescence microscopy imaging technology. The experimental results demonstrate that the anti-EGFR antibody conjugated FNs can effectively recognize breast cancer cells and exhibited good sensitivity and exceptional photostability, which would provide a novel way for the diagnosis and curative effect observation of breast cancer cells and offer a new method in detecting EGFR. |
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Keywords: | Anti-epidermal growth factor receptor (anti-EGFR) antibody Fluorescent nanoparticles (FNs) Breast cancer cell Fluorescence microscopy imaging |
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