| HPLC/MS测定动物组织及尿样中的β-兴奋剂 |
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| 引用本文： | 余建新,胡小钟,余纯,林雁飞,李亚飞,杨志学,颜怡,王鹏,李晶,张莉.HPLC/MS测定动物组织及尿样中的β-兴奋剂[J].分析测试技术与仪器,2004,10(4):244-247. |
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| 作者姓名： | 余建新 胡小钟 余纯 林雁飞 李亚飞 杨志学 颜怡 王鹏 李晶 张莉 |
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| 作者单位： | 湖北出入境检验检疫局技术中心,湖北,武汉,430022 |
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| 摘 要： | 采用微量化样品前处理技术，以固相萃取为净化方法．电喷雾正离子多反应监测方式建立了尿样及动物组织中β-兴奋剂残留量的液相色谱-串联质谱联用测定法。检测的定量限在0．15～0．6ng／g，线性范围均大于10^3，线性方程的相关系数大于0．999，组织样品和尿样的回收率大于70％．
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| 关 键 词： | 液相色谱-串联质谱 β兴奋剂 多反应监测 |
| 文章编号： | 1006-3757(2004)04-0244-04 |
| 收稿时间： | 2004/5/17 0:00:00 |
| 修稿时间： | 2004年5月17日 |
High-Performance Liquid Chromatography-Tandem Mass Spectrometry for the Determination of β-Agonist |
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| YU Jian-xin,HU Xiao-zhong,YU Chun,LIN Yan-fei,LI Ya-fei,YANG Zhi-xue,YAN Yi,WANG Peng,LI Jing and ZHANG Li.High-Performance Liquid Chromatography-Tandem Mass Spectrometry for the Determination of β-Agonist[J].Analysis and Testing Technology and Instruments,2004,10(4):244-247. |
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| Authors: | YU Jian-xin HU Xiao-zhong YU Chun LIN Yan-fei LI Ya-fei YANG Zhi-xue YAN Yi WANG Peng LI Jing and ZHANG Li |
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| Institution: | Technology Center of Hubei Entry-Exit Inspection and Quarantine Bureau, Wuhan 430022, China;Technology Center of Hubei Entry-Exit Inspection and Quarantine Bureau, Wuhan 430022, China;Technology Center of Hubei Entry-Exit Inspection and Quarantine Bureau, Wuhan 430022, China;Technology Center of Hubei Entry-Exit Inspection and Quarantine Bureau, Wuhan 430022, China;Technology Center of Hubei Entry-Exit Inspection and Quarantine Bureau, Wuhan 430022, China;Technology Center of Hubei Entry-Exit Inspection and Quarantine Bureau, Wuhan 430022, China;Technology Center of Hubei Entry-Exit Inspection and Quarantine Bureau, Wuhan 430022, China;Technology Center of Hubei Entry-Exit Inspection and Quarantine Bureau, Wuhan 430022, China;Technology Center of Hubei Entry-Exit Inspection and Quarantine Bureau, Wuhan 430022, China;Technology Center of Hubei Entry-Exit Inspection and Quarantine Bureau, Wuhan 430022, China |
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| Abstract: | The LC-MS-MS method is presented to analyze agonists residues in urine and animal tissue, using microchemical sample pretreatment technique combined with solid-phase extraction method. Analytes identification and quantification were performed using multiple reaction monitoring (MRM) with one precursor ion and three to four product ions as identifiers and electrospray ionization in positive mode. Limits of quantification were 0.15-0.6 ng/g for Clenbuterol and Saltbutamol in different samples; linear range were over 102, correlation coefficients of linear regression equations were above 0.999 and the recovery was over 70%. |
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| Keywords: | LC/MS/MS beta-agonist MRM |
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