16种手性化合物在不同自制手性固定相上的拆分比较

采用高效液相色谱法,在自制的纤维素-三(3,5-二甲基苯基氨基甲酸酯)(ATEO-OD)、纤维素-三(4-甲基苯基氨基甲酸酯)(ATEO-OG)和纤维素-三(4-甲基苯基甲酸酯)(ATEO-OJ)3种手性柱上对16种不同结构的手性化合物进行了拆分和比较.试验结果表明:16个手性样品在这3种手性固定相上分别获得了不同程度的拆分,A TEO-OD对所分析样品具有更好的手性识别能力,ATEO-OG和ATEO-OJ的手性识别能力相当.

Comparative Study of Chiral Separation of Sixteen Chiral Compounds on Cellulose-Based Chiral Stationary Phases

Three cellulose-based chiral columnscellulose tris(4-methylphenylcarbamate) (ATEO-OG), tris(3, 5-dimethylphenylcarbamate) (ATEO-OD), and tris(4-methyl benzoate) (ATEO-OJ)] were prepared in our laboratory. The comparasion and chiral separation of sixteen chiral compounds on the obtained cellulose-based chiral columns have been described. The effect of chromatographic conditions on chiral separation was investigated. The results showed that 16 chiral compounds were separated chirally with different degree on the three chiral stationary phases, and the chiral recognition ability of ATEO-OD was higher than the other two, baseline or partial enantioseparation of 16 compounds was obtained. ATEO-OG and ATEO-OJ has the same chiral recognition ability.