| meso-四-[4-(Boc-苏氨酸)氨基苯基]卟啉荧光探针对L-色氨酸的痕量测定 |
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| 引用本文： | 蔡燕燕,谭卫红,马艳,沈娟章,魏琴.meso-四-[4-(Boc-苏氨酸)氨基苯基]卟啉荧光探针对L-色氨酸的痕量测定[J].分析测试技术与仪器,2013,19(4):193-198. |
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| 作者姓名： | 蔡燕燕 谭卫红 马艳 沈娟章 魏琴 |
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| 作者单位： | 中国林业科学研究院 林产化学工业研究所, 生物质化学利用国家工程实验室, 国家林业局 林产化学工程重点开放性实验室, 江苏省生物质能源与材料重点实验室, 江苏 南京 210042;中国林业科学研究院 林产化学工业研究所, 生物质化学利用国家工程实验室, 国家林业局 林产化学工程重点开放性实验室, 江苏省生物质能源与材料重点实验室, 江苏 南京 210042;中国林业科学研究院 林产化学工业研究所, 生物质化学利用国家工程实验室, 国家林业局 林产化学工程重点开放性实验室, 江苏省生物质能源与材料重点实验室, 江苏 南京 210042;中国林业科学研究院 林产化学工业研究所, 生物质化学利用国家工程实验室, 国家林业局 林产化学工程重点开放性实验室, 江苏省生物质能源与材料重点实验室, 江苏 南京 210042;济南大学 化学化工学院, 山东 济南 250022 |
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| 基金项目： | 国家自然科学基金（20577016）和山东省自然科学基金（Y2008B44） |
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| 摘 要： | 利用荧光光谱方法探讨了以对称型pF3N子氨基酸卟啉：11R3S0-四-4-（Boc-苏氨酸）氨基苯基]卟啉（TAPP—Thr—Boc）作为光谱探针测定三一色氨酸的最佳条件．实验结果表明：双-2-乙基己基硫代琥珀酸钠（AOT）表面活性剂的加入能显著增加体系的灵敏度．在最佳实验条件下，体系荧光强度的下降程度与L-氨酸含量在0．10～8．80μg／mL范围内呈线性关系，检出限为0．034μg/mL方法抗干扰能力强，有较好的选择性和灵敏度，用于实际样品仔猪饲料的测定，结果满意．
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| 关 键 词： | 氨基酸卟啉 L-色氨酸 荧光光谱法 荧光探针 |
| 收稿时间： | 2013/9/24 0:00:00 |
| 修稿时间： | 2013/10/21 0:00:00 |
Determination of Trace Amounts of L-tryptophan Using Tetra-[(4-Bocthreonine)-aminophenyl]Porphyrin as Fluorescence Spectral Probe |
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| CAI Yan-yan,TAN Wei-hong,MA Yan,SHEN Juan-zhang and WEI Qin.Determination of Trace Amounts of L-tryptophan Using Tetra-[(4-Bocthreonine)-aminophenyl]Porphyrin as Fluorescence Spectral Probe[J].Analysis and Testing Technology and Instruments,2013,19(4):193-198. |
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| Authors: | CAI Yan-yan TAN Wei-hong MA Yan SHEN Juan-zhang and WEI Qin |
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| Institution: | Institute of Chemical Industry of Forestry Products, CAF, Key Laboratory of Biomass Energy and Material, Jiangsu Province, National Engineering Laboratory for Biomass Chemical Utilization, Key and Laboratory on Forest Chemical Engineering, SFA, Nanjing 210042, China;Institute of Chemical Industry of Forestry Products, CAF, Key Laboratory of Biomass Energy and Material, Jiangsu Province, National Engineering Laboratory for Biomass Chemical Utilization, Key and Laboratory on Forest Chemical Engineering, SFA, Nanjing 210042, China;Institute of Chemical Industry of Forestry Products, CAF, Key Laboratory of Biomass Energy and Material, Jiangsu Province, National Engineering Laboratory for Biomass Chemical Utilization, Key and Laboratory on Forest Chemical Engineering, SFA, Nanjing 210042, China;Institute of Chemical Industry of Forestry Products, CAF, Key Laboratory of Biomass Energy and Material, Jiangsu Province, National Engineering Laboratory for Biomass Chemical Utilization, Key and Laboratory on Forest Chemical Engineering, SFA, Nanjing 210042, China;School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China |
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| Abstract: | The optimum experimental conditions for the determination of L- tryptophan were investigated by fluorescence spectrophotometry using tetra- （4-Bocthreonine）-aminophenylI porphyrin as the fluorescence spectral probe. Experiments indicated that the sensitivity of the system can be enhanced significantly by the addition of bis（2-ethylhexyl）sulfosuecinate sodium salt （AOT）. Under optimum experimental conditions, the decreased of the fluorescence intensity of the system was in proportion to the concentration of L-tryptophan in the concentration range of 0.10 - 8.80 μg/mL with a limit of detection of 0. 034 μg/mL. The method has been used in the determination of samples with satisfactory results. |
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| Keywords: | amino-acid porphyrin|L-tryptophan|fluorescent spectrometry|fluorescent probe |
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