| 钙黄绿素-藏红T与DNA作用机理的光谱研究 |
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| 引用本文： | 林旭聪,谢增鸿,李嘉倩,郭良洽,陈国南.钙黄绿素-藏红T与DNA作用机理的光谱研究[J].分析测试技术与仪器,2003,9(3):147-151. |
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| 作者姓名： | 林旭聪 谢增鸿 李嘉倩 郭良洽 陈国南 |
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| 作者单位： | 福州大学化学化工学院化学系,福建,福州,350002 |
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| 基金项目： | 国家教育部《高等学校骨干教师资助计划》，福建省科技攻关计划 (2 0 0 2Y0 51 )，福州大学科技发展基金 (2 0 0 2 -XQ -1 1 )等资助项目 |
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| 摘 要： | 应用能量转移方法和光谱技术，研究了钙黄绿素-藏红T荧光能量转移体系与ctDNA的作用机理以及抑制作用的影响因素．在pH8．0和低离子强度条件下，ctDNA与Caleein-ST发生了静电电荷作用，降低了钙黄绿素-藏红T荧光体系的能量转移效率，对荧光体系的能量转移产生了明显的抑制作用．
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| 关 键 词： | 钙黄绿素 藏红T DNA 作用机理 光谱研究 能量转移 抗癌药物 |
| 文章编号： | 1006-3757(2003)03-0147-05 |
| 收稿时间： | 2003/4/14 0:00:00 |
| 修稿时间： | 2003年4月14日 |
Spectrophotometric Studies on the Interaction Mechanism of Calcein-Safranine T Fluorescence Pair with DNA |
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| LIN Xu-cong,XIE Zeng-hong,LI Jia-qian,GUO Liang-qia and CHEN Guo-nan.Spectrophotometric Studies on the Interaction Mechanism of Calcein-Safranine T Fluorescence Pair with DNA[J].Analysis and Testing Technology and Instruments,2003,9(3):147-151. |
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| Authors: | LIN Xu-cong XIE Zeng-hong LI Jia-qian GUO Liang-qia and CHEN Guo-nan |
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| Institution: | Department of Chemistry, Fuzhou University, Fuzhou 350002, China;Department of Chemistry, Fuzhou University, Fuzhou 350002, China;Department of Chemistry, Fuzhou University, Fuzhou 350002, China;Department of Chemistry, Fuzhou University, Fuzhou 350002, China;Department of Chemistry, Fuzhou University, Fuzhou 350002, China |
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| Abstract: | Via spectrophotometric method and fluorescence transfer system, the reaction mechanism of Calcein-Safranine T with calf thymus DNA(ctGNA) was investigated. Factors on the inhibition of this fluorescence transfer system were also discussed. At pH 8.0 and low ionic strength, the static electric charge interaction of calf thymus DNA with Safranine T occurred and resulted in remarkable inhibition of the fluorescence energy transfer of Calcein-Safranine T pair. |
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| Keywords: | mechanism investigation energy transfer Calcein- Safranine T |
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