| 液相色谱-电喷雾电离/离子阱质谱法分析金线莲中3-吡啶甲醇 |
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| 引用本文： | 黄可可,黄丽英,吴萍萍,陈圆婷,周伟炜.液相色谱-电喷雾电离/离子阱质谱法分析金线莲中3-吡啶甲醇[J].分析测试技术与仪器,2011,17(3):129-133. |
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| 作者姓名： | 黄可可 黄丽英 吴萍萍 陈圆婷 周伟炜 |
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| 作者单位： | 福建医科大学 药学院,福建 福州,350004 |
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| 基金项目： | 福建省科技厅重点项目(2009I0009)，福建医科大学教授基金项目(01601086). |
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| 摘 要： | 应用现代提取技术超声-微波协同萃取金线莲中的3-吡啶甲醇,采用固相萃取技术对样品进行前处理,高效液相色谱法-电喷雾电离/离子阱质谱法(HPLC-ESI/MS)对提取物中3-吡啶甲醇进行测定和鉴别.色谱条件:Agilent TC-C18色谱柱(5μm,4.6×250 mm),流动相:甲醇-0.02 mol/L乙酸铵(5∶...
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| 关 键 词： | 金线莲 固相萃取 液-质联用 3-吡啶甲醇 |
| 收稿时间： | 2011/7/11 0:00:00 |
| 修稿时间： | 2011/8/16 0:00:00 |
Analysis of 3-pyridinemethanol in Anoectochilus Roxburghii by Liquid Chromatography-Electrospray Ion Trap Mass Spectrometry |
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| HUANG Ke-ke,HUANG Li-ying,WU Ping-ping,CHEN Yuan-ting and ZHOU Wei-wei.Analysis of 3-pyridinemethanol in Anoectochilus Roxburghii by Liquid Chromatography-Electrospray Ion Trap Mass Spectrometry[J].Analysis and Testing Technology and Instruments,2011,17(3):129-133. |
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| Authors: | HUANG Ke-ke HUANG Li-ying WU Ping-ping CHEN Yuan-ting and ZHOU Wei-wei |
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| Institution: | College of Pharmacy, Fujian Medical University, Fuzhou 350004,China;College of Pharmacy, Fujian Medical University, Fuzhou 350004,China;College of Pharmacy, Fujian Medical University, Fuzhou 350004,China;College of Pharmacy, Fujian Medical University, Fuzhou 350004,China;College of Pharmacy, Fujian Medical University, Fuzhou 350004,China |
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| Abstract: | 3-pyridinemethanol was extracted from Anoectochilus roxburghii by the ultrasonic-microwave assisted extraction(UMAE).After pretreatment using solid phase extraction(SPE) technology,3-pyridinemethanol was identified and determined by high performance liquid chromatography-electrospray ion traps mass spectrometry.The chromatographic separation was achieved on an Agilent TC-C18 column(5 μm,4.6×250 mm)using methanol-0.02 mol/L ammonium acetate(5∶ 95,V/V)as the mobile phase at a flow rate of 1.0 mL/min,and 260 nm as the detection wavelength.The analyte showed good linear regression relationship between peak area and concentrations in the range of 1～0 μg/mL(r = 0.9996).The recovery rate was 92.0%～ 96.2% with an RSD of 2.36%.The proposed method is simple,accurate and rapid. |
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| Keywords: | anoectochilus roxburghii SPE LC-MS 3-pyridinemethanol |
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