| 气相色谱-质谱法测定鸡蛋中三聚氰胺 |
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| 引用本文： | 杨晓凤,毛建菲,胡莉.气相色谱-质谱法测定鸡蛋中三聚氰胺[J].分析测试技术与仪器,2013,19(2):99-102. |
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| 作者姓名： | 杨晓凤 毛建菲 胡莉 |
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| 作者单位： | 四川省农业科学院分析测试中心,四川成都,610066 |
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| 摘 要： | 建立了气相色谱-质谱法测定鸡蛋中三聚氰胺的方法.鸡蛋样品经三氯乙酸提取,乙酸铅沉淀蛋白后,过MCX阳离子交换固相萃取小柱除去样品基质干扰,用N,O-双三甲基硅基三氟乙酰胺(BSTFA)+三甲基氯硅烷(TMCS)(99+1)衍生,进行气相色谱-质谱分析.在0.010～2.00 mg/L浓度范围内,线性关系良好(r=0.9996),检出限为0.01 mg/kg.方法回收率为90.1%～94.7%,RSD为1.7%～5.3%.方法准确、灵敏,适用于鸡蛋中三聚氰胺的检测.
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| 关 键 词： | 三聚氰胺 鸡蛋 气相色谱-质谱 |
| 收稿时间： | 2012/12/28 0:00:00 |
| 修稿时间： | 4/8/2013 12:00:00 AM |
Determination of Melamine in Eggs by Gas Chromatography-Mass Spectrometry |
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| YANG Xiao-feng,MAO Jian-fei and HU Li.Determination of Melamine in Eggs by Gas Chromatography-Mass Spectrometry[J].Analysis and Testing Technology and Instruments,2013,19(2):99-102. |
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| Authors: | YANG Xiao-feng MAO Jian-fei and HU Li |
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| Institution: | Center of Analysis and Testing, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China;Center of Analysis and Testing, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China;Center of Analysis and Testing, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China |
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| Abstract: | A gas chromatographic-mass spectrometric method for the quantitative analysis of melamine in eggs has been developed. The sample was extracted by trichloroacetic acid, settled by lead acetate for the protein precipitation, cleaned-up by the MCX cation exchange solid phase extraction column, derivatized with BSTFA+TMCS (99+1) and processed for analysis by the gas chromatography-mass spectrometry. The calibration curves showed good linearity in the range of 0.010~2.00 mg/L for melamine with a correlation coefficent of 0.9996. The limit of detection was 0.01 mg/kg. The recoveries were 90.1%-94.7% and relative standard deviations were 1.7%-5.3%. The method is accurate and sensitive for the determination of melamine in eggs. |
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| Keywords: | melamine chicken egg GC-MS |
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