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| HPLC-DAD分析金线莲中天麻素和天麻苷元 | |
| 引用本文： | 陈惠芳,吴萍萍,黄丽英,陈瑜,林守二.HPLC-DAD分析金线莲中天麻素和天麻苷元[J].分析测试技术与仪器,2015(2):69-76. |
| 作者姓名： | 陈惠芳 吴萍萍 黄丽英 陈瑜 林守二 |
| 作者单位： | 1. 泉州医学高等专科学校,福建 泉州,362000 2. 福建医科大学 药学院,福建 福州,350004 3. 福建医科大学 基础医学院,福建 福州,350004 |
| 基金项目： | 国家卫生和计划生育委员会科研基金课题(wkj-FJ-33),福建省自然基金项目(2012J01363). |
| 摘 要： | 研究超声-微波协同法提取金线莲中的天麻素和天麻苷元,并对提取条件进行了优化.应用高效液相-二极管阵列检测法分析提取物中的天麻素和天麻苷元.研究结果表明:金线莲中天麻素、天麻苷元和其他杂质成分能够很好的分离,且两者的含量分别为1.430及0.562 mg/g,相对标准偏差为0.94%和0.25%.方法准确、快速、灵敏度高、重现性好. |
| 关 键 词： | 金线莲 天麻素 天麻苷元 超声-微波提取 高效液相-二极管阵列检测法 |
| 收稿时间： | 2015/5/16 0:00:00 |
| 修稿时间： | 2015/6/24 0:00:00 |
Analysis of Gastrodine and 4-hydroxybenzyl Alcohol in A.roxburghii by HPLC-DAD |
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| CHEN Hui-fang,Wu Pin-pin,HUANG Li-ying,CHEN Yu and LIN Shou-er.Analysis of Gastrodine and 4-hydroxybenzyl Alcohol in A.roxburghii by HPLC-DAD[J].Analysis and Testing Technology and Instruments,2015(2):69-76. | |
| Authors: | CHEN Hui-fang Wu Pin-pin HUANG Li-ying CHEN Yu and LIN Shou-er |
| Institution: | Department of Pharmacy, Quanzhou Medical College, Quanzhou 362000, China;College of Pharmacy, Fujian Medical University, Fuzhou 350004, China;College of Pharmacy, Fujian Medical University, Fuzhou 350004, China;College of Preclinical Medicine Fujian Medical University, Fuzhou 350004, China;College of Pharmacy, Fujian Medical University, Fuzhou 350004, China |
| Abstract: | The ultrasound-microwave assisted extraction (UMAE) method has been applied to the extraction of Anoectocilus roxburghii(A.roxburghii) and the extraction conditions were optimized in this study. A method of HPLC-DAD was developed for the simultaneous identification and determination of gastrodine and 4-hydroxybenzyl alcohol. Results showed that the contents of gastrodine and 4-hydroxybenzyl alcohol from A.roxburghii were 1.430 mg/g and 0.562 mg/g, and relative standard deviations were 0.94% and 0.25% respectively. The proposed method is simple, accurate, rapid and reproducible. |
| Keywords: | Anoectocilusroxburghii(A roxburghii) gastrodine 4-hydroxybenzylalcohol UMAE HPLC-DAD |
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