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| 市售大黄及三黄片中蒽醌类活性成分的胶束电动毛细管色谱含量测定 | |
| 引用本文： | 沈守杰,岳美娥,师彦平.市售大黄及三黄片中蒽醌类活性成分的胶束电动毛细管色谱含量测定[J].分析测试技术与仪器,2005,11(2):90-94. |
| 作者姓名： | 沈守杰 岳美娥 师彦平 |
| 作者单位： | 1. 兰州大学,功能有机分子国家重点实验室,兰州大学,化学化工学院,甘肃,兰州,730000 2. 中国科学院,兰州化学物理研究所,甘肃省天然药物重点实验室,甘肃,兰州,730000 3. 兰州大学,功能有机分子国家重点实验室,兰州大学,化学化工学院,甘肃,兰州,730000;中国科学院,兰州化学物理研究所,甘肃省天然药物重点实验室,甘肃,兰州,730000 |
| 基金项目： | 兰州大学校科研和教改项目 |
| 摘 要： | 建立了胶束电动毛细管色谱分离和测定大黄及其制剂三黄片中蒽醌类活性组分的方法．考察了背景电解质pH、表面活性剂浓度、有机改性剂种类和浓度对分离的影响．实验结果表明：在缓冲液pH为9．5、SDS浓度为25mmol／L、乙氰浓度为20％时的优化条件下，大黄及三黄片中蒽醌类活性组分得到基线分离且方法具有较好的重现性． |
| 关 键 词： | 蒽醌 大黄 毛细管胶束电动色谱 |
| 文章编号： | 1006-3757(2005)02-0090-05 |
| 收稿时间： | 2004/12/20 0:00:00 |
| 修稿时间： | 2004年12月20 |
Separation and Determination of Anthraquinones in Rhubarb and its Preparation by Micellar Electrokinetic Capillary Chromatography |
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| SHEN Shou-jie,YUE Mei-e and SHI Yan-ping.Separation and Determination of Anthraquinones in Rhubarb and its Preparation by Micellar Electrokinetic Capillary Chromatography[J].Analysis and Testing Technology and Instruments,2005,11(2):90-94. | |
| Authors: | SHEN Shou-jie YUE Mei-e and SHI Yan-ping |
| Institution: | State Key Laboratory of Functional Organic Molecule, College of Chemistry and Chemical Engineering Lanzhou University, Lanzhou 730000, China;Key Laboratory for Naturel Medicine of Gasn Province, Lanzhou Institute of Chemical Physics, the Chinese Academy of Sciences, Lanzhou 730000, China;State Key Laboratory of Functional Organic Molecule, College of Chemistry and Chemical Engineering Lanzhou University, Lanzhou 730000, China;Key Laboratory for Naturel Medicine of Gasn Province, Lanzhou Institute of Chemical Physics, the Chinese Academy o |
| Abstract: | A micellar electrokinetic capillary chromatography method was developed for the determination of anthraquinones in rhubarb and its preparation. The effects of pH value of buffer system, the concentration of sodium dodecyl sulfate (SDS), the organic modifier and its concentration on the separation selectivity were discussed. The results indicated that the anthraquinones were baseline separation under the 10 mmol/L borate buffer at pH 9.5 with 25 mmol/L SDS and 20%(V/V) acetonitrile. The calibration curves exhibited good linearity between the concentrations and the peak area of the three components. The relative standard deviations (RSD) of migration time and peak areas were <3.45% and 4.86%, respectively. |
| Keywords: | Anthraquinones Rhubarb Micellar electrokinetic capillary chromatography |
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