| 一种近红外荧光探针的合成、表征及应用 |
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| 引用本文： | 张逢源,刘晓燕,叶为春,张海霞.一种近红外荧光探针的合成、表征及应用[J].分析测试技术与仪器,2019,25(4):228-231. |
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| 作者姓名： | 张逢源 刘晓燕 叶为春 张海霞 |
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| 作者单位： | 兰州大学 化学化工学院,甘肃 兰州 730000;宁夏医科大学 基础医学院,宁夏 银川 750004;兰州大学 化学化工学院,甘肃 兰州,730000 |
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| 基金项目： | 国家自然科学基金（No.21575055）和兰州大学教务处教学项目（No.2018057） |
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| 摘 要： | 合成了一种卟啉类小分子荧光探针5,10,15,20-四对羟苯基卟啉(THPP)并对其结构进行表征.通过荧光光谱法,可以分别定性、定量检测铜离子(Cu2+)、焦磷酸根(PPi)及碱性磷酸酶(ALP).研究结果可以转化为一个代表性综合实验,激发学生对科学研究的极大兴趣,培养学生利用综合知识的能力.实验囊括了有机化学、分析化学、生物化学及无机化学等多个学科的多个知识点及多种重要的仪器操作技术,有助于学生综合实验能力的提升.
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| 关 键 词： | 近红外荧光探针 多组分检测 综合化学实验 |
| 收稿时间： | 2019/9/26 0:00:00 |
| 修稿时间： | 2019/11/2 0:00:00 |
Synthesis and Characterization of a NIR Fluorescent Sensor for Its Application in Multicomponent Detection |
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| ZHANG Feng-yuan,LIU Xiao-yan,YE Wei-chun and ZHANG Hai-xia.Synthesis and Characterization of a NIR Fluorescent Sensor for Its Application in Multicomponent Detection[J].Analysis and Testing Technology and Instruments,2019,25(4):228-231. |
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| Authors: | ZHANG Feng-yuan LIU Xiao-yan YE Wei-chun and ZHANG Hai-xia |
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| Institution: | College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China;School of Basic Medical Science, Ningxia Medical University, Yinchuan 750004, China,College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China,College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China |
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| Abstract: | A comprehensive chemical experiment for college students was introduced through the synthesis and characterization of a porphyrin NIR fluorescent sensor, 5,10,15,20-Tetrakis(4-hydroxyphenyl) porphyrin (THPP), and its application in multicomponent detection of Cu2+, pyrophosphate (PPi) and alkaline phosphatase (ALP). The probe was characterized carefully. According to this experiment, students'' great interest in scientific research can be stimulated, and research capability can be cultivated. Furthermore, this experiment involves the knowledge of organic chemistry, analytical chemistry, instrumental analysis and inorganic chemistry, which is conducive to improving students'' comprehensive abilities. |
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| Keywords: | NIR fluorescent sensor multicomponent detection comprehensive chemical experiment |
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