| 一种液相色谱分离与质谱鉴定白血病K562细胞蛋白的方法 |
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| 引用本文： | 陈曼,王骊丽,耿信笃.一种液相色谱分离与质谱鉴定白血病K562细胞蛋白的方法[J].分析测试技术与仪器,2007,13(2):98-101. |
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| 作者姓名： | 陈曼 王骊丽 耿信笃 |
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| 作者单位： | 西北大学,现代分离科学研究所,现代分离科学陕西省重点实验室,陕西,西安,710069 |
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| 摘 要： | 分别通过3种色谱模式:反相高效液相色谱(RPLC)、弱阴离子交换-反相高效液相色谱(WAX-RPLC)和排阻-反相高效液相色谱(SEC-RPLC)对K562细胞的蛋白质进行分离,收集的色谱馏分采用基质辅助电离解析时间飞行质谱(MALDI-TOF MS)进行鉴定后,比较所获得的蛋白质数据.结果显示:当选择SEC-RPLC模式构建K562细胞蛋白质图谱时,检测到的蛋白质数目最多,信息最为全面.此法的建立为白血病的临床研究提供了一种有效的手段.
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| 关 键 词： | 高效液相色谱 基质辅助电离解析时间飞行质谱 K562细胞 蛋白质组 |
| 文章编号： | 1006-3757（2007）02-0098-04 |
| 收稿时间： | 2006-11-30 |
| 修稿时间： | 2006-11-302007-01-29 |
A Liquid Separation and MALDI-TOF MS Detection Method for Leukemia K562 Cell |
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| CHEN Man,WANG Li-li and GENG Xin-du.A Liquid Separation and MALDI-TOF MS Detection Method for Leukemia K562 Cell[J].Analysis and Testing Technology and Instruments,2007,13(2):98-101. |
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| Authors: | CHEN Man WANG Li-li and GENG Xin-du |
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| Abstract: | The proteins of K562 cell line were separated by three modes of chromatography,the chromatographic fractions were collected and then detected by MALDI-TOF MS.From comparing the date of those separation modes a conclusion can be made: Using SEC-RPLC separation mode to establish the protein finger print of K562 cell line,more than 120 kinds of proteins from 2 KD to 130 KD can be detected.The detected proteins are the most and the required information is also most comprehensive by using this separation mode.The establishing of this method provides a valid way for clinical study of leukemia. |
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| Keywords: | high performance liquid chromatography(HPLC) matrix assisted laser desorption/ionization-time of flight mass spectrometry(MALDI-TOF MS) K562 cell line proteome |
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