| Abstract: | A rapid method has been developed for rapid sample clean-up in the determination of the pharmocologically active terpenoid including ginkgolide A, B. C and bilobalide in ginkgo biloba leaves extracts (GBE). The extracts are dissolved in 7% of ethanol aqueous solution and then purified by a highly selective polymeric absorbent solid-phase chromatographic column. After being concentrated, the separated terpenoids with no phenolic disturbance are determined by highperformance liquid chromatography on a Nova-Pak C18 column with methanol-water (30:70) as effluent and refractive index defection. The recovery of the method is about 95% and the new method saves more time than the conventional two-column purification method. |
