Purified 14C Virginiamycin

Abstract

¹⁴C virginiamycin, an antibiotic composed of two synergistic factors S and M, was required for metabolism and residue studies. ¹⁴C Factors S and M were separated from crude ¹⁴C virginiamycin and purified by semi-preparative normal phase HPLC followed by crystallization/precipitation. After chemical and radiopurity assays, the two factors were recombined in a 1:4 ratio to form purified ¹⁴C virginiamycin.