Separation,optimization, and quantification of cytokinins by a recently developed amide-embedded stationary phase |
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Authors: | Hayriye Aral Duygu Haşimi Abdulkadir Levent Berrin Ziyadanoğullari |
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Institution: | 1. Department of Chemistry, Faculty of Science and Art, University of Batman, Batman, Turkey;2. Department of Chemistry, Faculty of Science, University of Dicle, Diyarbak?r, Turkey |
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Abstract: | In this study, some plant growth regulators known as cytokinins kinetin (K), zeatin (Z), thidiazuron (TDZ), benzylaminopurine (BAP), and dimethylallylaminopurine (AAP)] were separated by HPLC using an amide-embedded mixed-mode stationary phase which was synthesized by Aral et al. in recent years. The effect of mobile phase content, mobile phase pH, buffer concentration, and temperature on separation process was studied. In addition, a quantitative determination of cytokinins from Salvia limbata extract was studied, and some validation parameters such as limit of detection (LOD), limit of quantification (LOQ), and relative standard deviation (RSD) were calculated as a range of 0.03–0.1, 0.1–0.26?mg/L, and 0.03–0.08, respectively. |
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Keywords: | Amide-embedded stationary phase cytokinins mixed-mode stationary phase plant growth regulators Salvia limbata |
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