Determination and quantification of phenolic compounds in methanolic extracts of Solanum ferrugineum (Solanaceae) fruits by HPLC-DAD and HPLC/ESI-MS/TOF |
| |
Authors: | José Roberto Medina-Medrano María Daniela Mares-Quiñones Juan Ignacio Valiente-Banuet Monserrat Vázquez-Sánchez Dioselina Álvarez-Bernal Edgar Villar-Luna |
| |
Institution: | 1. CONACYT—Instituto Politécnico Nacional, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional Unidad Michoacán, Jiquilpan, Michoacán, Méxicojrmedina@conacyt.mx;3. Escuela de Ingeniería y Ciencias, Centro de Biotecnología FEMSA, Tecnologico de Monterrey, Monterrey, Nuevo León, Mexico;4. CONACYT—Instituto Politécnico Nacional, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional Unidad Michoacán, Jiquilpan, Michoacán, México;5. Instituto Politécnico Nacional, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional, Unidad Michoacán, Jiquilpan, Michoacán, México |
| |
Abstract: | Solanum ferrugineum Jacq. is a wild species that in previous analysis reported a significant antioxidant capacity. The aim of our research was to determine total phenolic content (TPC) and total flavonoid content (TFC), and the phenolic composition by HPLC-DAD and HPLC/ESI-MS/TOF of methanolic extracts of S. ferrugineum fruits, collected from Paredones, Jiquilpan, and Fray Dominguez, Pajuacarán in the Mexican state of Michoacán. TPC and TFC were determined by the spectrophotometric Folin–Ciocalteu reagent and the AlCl3 method, respectively. TPC in S. ferrugineum fruit 31.41?±?0.91?mg gallic acid equivalent/g dry tissue (DT)] was similar to those reported for Turkey berry (Solanum torvum Sw.) and eggplant (Solanum melongena L.) fruits. The TFC values of S. ferrugineum fruits (29.14?±?4.99?mg catechin equivalent /g DT) corresponded to 80.24% of the TPC. Eight phenolic compounds (PC) were identified by HPLC analysis. The main PC identified in S. ferrugineum fruits were chlorogenic acid, caffeic acid, p-coumaric acid, gallic acid, quercetin, and kaempferol. S. ferrugineum fruits could be used as a starting material for the extraction of high-value PC with potential applications. |
| |
Keywords: | Cherry eggplant flavonoids HPLC phenolic profile Solanum ferrugineum wild plant |
|
|