| A NOVEL MOLECULARLY IMPRINTED POLYMER： SYNTHESIS AND ADSORPTION BEHAVIOR OF GATIFLOXACIN |
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| 作者单位： | GE Yanru,MO Chuanjie,CHEN Zhixian(College of Pharmacy,Jiangsu University,Zhenjiang 212013,Jiangsu,China);YAN Yongsheng,XU Wanzhen(College of Chemistry and Chemical Engineering,Jiangsu University Zhenjiang 212013,Jiangsu,China) |
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| 基金项目： | 国家科技基金，Jiangsu University Science Research Funding |
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| 摘 要： | A kind of molecularly imprinted polymer （MIPs） with high selectivity was prepared using methacrylic acid （MAA） as functional monomer, ethylene glycol dimethacrylate （EDMA） as crosslinker and Gatifloxacin as template. The effect of various parameters such as volume of solvent, functional monomer dosage, crosslinker dosage and polymerization time were investigated. The selective binding experiment for substrates show that the affinity and selectivity for Gatifloxacin were higher than that for blank polymer. Scatchard analysis show that the MIPs recognized template with two kinds of binding sites. The dissociation constant Kd and maximum adsorption quantity Qmax of these two kinds of binding sites were calculated： Kd1 and Qmax1 of the binding sites with high affinity were 8.67×10^-4 mol/L and 28.19μmol/g, while Kd2 and Qmax2 of the binding sites with low affinity were 1.05×10^-3 mol/L and 33.20μmol/g respectively.
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| 关 键 词： | 高分子聚合物 斯卡查德分析 联系种 EDMA |
A NOVEL MOLECULARLY IMPRINTED POLYMER:SYNTHESIS AND ADSORPTION BEHAVIOR OF GATIFLOXACIN |
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| Authors: | GE Yanru MO Chuanjie CHEN Zhixian YAN Yongsheng XU Wanzhen |
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| Institution: | 1. College of Pharmacy,Jiangsu University,Zhenjiang 212013,Jiangsu,China
2. College of Chemistry and Chemical Engineering,Jiangsu University Zhenjiang 212013,Jiangsu,China |
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| Abstract: | A kind of molecularly imprinted polymer (MIPs) with high selectivity was prepared using methacrylic acid (MAA) as functional monomer, ethylene glycol dimethacrylate (EDMA) as crosslinker and Gatifloxacin as template.The effect of various parameters such as volume of solvent, functional monomer dosage, crosslinker dosage and polymerization time were investigated.The selective binding experiment for substrates show that the affinity and selectivity for Gatifloxacin were higher than that for blank polymer.Scatchard analysis show that the MIPs recognized template with two kinds of binding sites.The dissociation constant Kd and maximum adsorption quantity Qmax of these two kinds of binding sites were calculated: Kd1 and Qmax1 of the binding sites with high affinity were 8.67×10-4mol/L and 28.19μmol/g, while Kd2 and Qmax2 of the binding sites with low affinity were 1.05×10-3 mol/L and 33.20μmol/g respectively. |
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| Keywords: | Gatifloxacin Molecularly imprinted polymer Scatchard analysis Binding sites |
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