LC-MS/MS methods for determination of unstable pivmecillinam and mecillinam in acidified human plasma: Application to a pharmacokinetic study |
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Authors: | Lu-Ning Sun Yang Zhao Hua-Ye Gao Yan-Ling Yang Xu-Ping Qian Shi-Yu Sun Yu Wang Li Ding Yong-Qing Wang |
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Institution: | 1. Research Division of Clinical Pharmacology, First Affiliated Hospital of Nanjing Medical University, Nanjing, P. 2. R. 3. China
School of Pharmacy, Nanjing Medical University, Nanjing, P. R. China;4. China;5. Department of Pharmaceutical Analytical, China Pharmaceutical University, Nanjing, P. R. China |
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Abstract: | Pivmecillinam, the ester of biologically active antibiotic mecillinam, is an effective oral preparation to treat urinary tract infections. To study pharmacokinetics in humans, LC-MS/MS methods were developed to quantify pivmecillinam and mecillinam in human plasma, respectively. Considering cephalexin as internal standard, analytes were separated on UltimateXB-C18 columns after protein precipitation by acetonitrile. The mobile phase was composed of water containing 0.1% formic acid and methanol. The multiple reactions monitoring transitions of m/z 440.2→167.1, 326.1→167.1, and 348.1→158.1 were selected to inspect pivmecillinam, mecillinam, and the internal standard in positive ion mode. No apparent matrix effect was perceived. Linearities were obtained over calibration ranges of 0.0500–12.0 and 10.0–15,000 ng/mL, respectively. The intraday precisions were below 5.5%, the interday precisions were below 6.1%, and accuracies were within –8.1 to 13.0%. Stability tests were conducted and an acidification step was explored to enhance the stability of pivmecillinam and mecillinam. Further stability was validated under various storage and processing conditions. Both methods were applied to a pharmacokinetic study of pivmecillinam and mecillinam after oral administration of 400 mg pivmecillinam hydrochloride tablets in healthy Chinese subjects. |
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Keywords: | human plasma mecillinam pharmacokinetics pivmecillinam tandem mass spectrometry |
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