Measurement of resistant starch |
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Authors: | McCleary Barr V Monaghan Dympna A |
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Affiliation: | Megazyme International Ireland Ltd, Bray, County Wicklow. barry@megazyme.com |
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Abstract: | A robust and reliable method was developed to measure resistant starch (RS), i.e., starch that enters the large intestine. In vivo conditions were reflected as much as possible while a user-friendly format was maintained. Parameters investigated included a-amylase concentration, pH of incubation, maltose inhibition of alpha-amylase, the need for amyloglucosidase inclusion, the effect of shaking and stirring on determined values, and problems in recovering and analyzing the RS-containing pellet. The RS values obtained were in good agreement with published in vivo data. An interlaboratory evaluation of the method has been completed (First Action Method 2002.02). |
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