基于双层纳米金修饰的高灵敏电位型乙肝表面抗原免疫传感器研究

基于电沉积和层层自组装技术，提出了一种新的生物分子固定化方法，研制成一种高灵敏电位型乙肝表面抗原免疫传感器。利用L-半胱胺酸（LCys）的双官能团结合双层纳米金，从而通过比表面积大，生物相容性好的纳米金胶吸附大量抗体，同时用聚乙烯醇缩丁醛（PVB）薄膜的笼效应把乙肝表面抗体（HBsAb）和纳米金固定在玻碳电极上，从而制得了高灵敏度、高稳定性的电位型免疫传感器。采用循环伏安法（CV）对电极的层层自组装过程进行了考察，并对该免疫传感器的性能进行了详细的研究。该免疫传感器线性范围是8．5～256．0ng／mL，线性相关系数为0．9978，灵敏度为89．0，检出限为3．1ng／mL。已用于病人的血清样品分析。

Highly sensitive potentiometric immunosensor for hepatitis B surface antigen based on double-layer gold nanoparticles

A novel potentiometric immunosensor for the detection of hepatitis B surface antigen(HBsAg) has been developed by means of electrodeposition and self-assembly techniques.L-cysteine was combined with bilayer gold nanoparticle by its-SH and-NH_2 group.A large amount of hepatitis B surface antibody(HBsAb) was adsorbed by gold nanoparticle and embeded in the PVB firmly because of cage effect of polyvinyl butyral(PVB).Then a potentiometric immunosensor with good sensitivity and stability was prepared.The characteristics of the modified electrode at different stages of modification were studied by cyclic voltammetry(CV).The resulting immunosensor exhibits wide linear range from 8.5 to 256.0 ng/mL with a detection limit of 3.1 ng/mL.The immunosensor was used to analyze the serum samples with satisfactory results.