| 壳聚糖-二茂铁复合物的制备及其在电化学传感器上的应用 |
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| 引用本文： | 焦守峰,沈荣星,方宾.壳聚糖-二茂铁复合物的制备及其在电化学传感器上的应用[J].分析试验室,2009,28(6). |
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| 作者姓名： | 焦守峰 沈荣星 方宾 |
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| 作者单位： | 安徽师范大学化学与材料科学学院,芜湖,241000 |
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| 摘 要： | 壳聚糖-二茂铁复合物(CHIT-Fc)由二茂铁羧酸的羧基和脱乙酰壳多糖的羟基缩合合成,并通过红外光谱检测.合成得到的壳聚糖-二茂铁复合物通过物理吸附作为固定胆固醇氧化酶(CHOx)的基体.同时,使用Nafion稀释液可以消除诸如抗坏血酸和尿酸的影响.最佳测试条件下,采用示差脉冲(DPV)研究胆固醇生物传感器的响应,在4.0×10-6mol/L～1.0×10-4 mol/L范围内,氧化峰电流与胆固醇浓度呈现良好的线性关系,线性方程为Ipa=0.0223c-0.0875(Ipa:μA,c:μmol/L,R=0.9982),检测限为5.0×10-7mol/L(S/N=3).
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| 关 键 词： | 壳聚糖-二茂铁复合物 胆固醇氧化酶 生物电催化氧化 传感器 |
Preparation of ferrocene-containing chitosan derivative and its application to enzyme-based electrochemical biosensors |
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| JIAO Shou-feng,SHEN Rong-xing,FANG Bin.Preparation of ferrocene-containing chitosan derivative and its application to enzyme-based electrochemical biosensors[J].Chinese Journal of Analysis Laboratory,2009,28(6). |
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| Authors: | JIAO Shou-feng SHEN Rong-xing FANG Bin |
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| Institution: | College of Chemistry and Materials Science;Anhui Normal University;Wuhu 241000 |
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| Abstract: | Ferrocene-containing chitosan derivative(CHIT-Fc) is synthesized by hydroxyl groups of chitosan with ferrocenecarboxylic acid.The structure of the product is determined by FT-IR spectrometry.CHIT-Fc is used as a functionalized matrix to immobilize cholesterol oxidase(ChOx) by physical adsorption.This redox polysaccharide not only provides a suitable environment for enzyme entrapment but also establishes efficient electrical communication between ChOx and the electrodes.Also,it could make this enzyme electro... |
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| Keywords: | Ferrocene-containing chitosan Cholesterol oxidase Bioelectrocatalytic oxidation Biosensor |
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