| TiO_2溶胶二级散射光谱法测定人血清白蛋白 |
| |
| 引用本文： | 梁丽娜,穆志英,董存智.TiO_2溶胶二级散射光谱法测定人血清白蛋白[J].分析试验室,2010,29(2). |
| |
| 作者姓名： | 梁丽娜 穆志英 董存智 |
| |
| 作者单位： | 淮北煤炭师范学院化学系,淮北,235000 |
| |
| 摘 要： | 制备了TiO2溶胶,并通过透射电子显微镜等对其结构进行了表征。研究了TiO2溶胶与人血清白蛋白(HSA)的相互作用。基于HSA对TiO2溶胶二级散射峰的增强作用,建立了二级散射光谱法测定痕量白蛋白的新方法。方法的线性范围是0.005~1.5 mg/L,检出限为3.5μg/L。方法用于人血中HSA的测定,回收率为98%~100.2%。
|
| 关 键 词： | TiO2溶胶 二级散射 人血清白蛋白 |
Determination of human serum albumin by double scattering of TiO_2 sol |
| |
| LIANG Li-na,MU Zhi-ying,DONG Cun-zhi.Determination of human serum albumin by double scattering of TiO_2 sol[J].Chinese Journal of Analysis Laboratory,2010,29(2). |
| |
| Authors: | LIANG Li-na MU Zhi-ying DONG Cun-zhi |
| |
| Institution: | College of Chemistry and Material Science;Huaibei Coal Teachers College;Huaibei 235000 |
| |
| Abstract: | TiO_2 collosol was prepared, and the structure of the TiO_2 collosol was characterized by TEM. The Interaction of TiO_2 collosol with Human serum albumin (HSA) was studied. The TiO_2 collosol could be used as a double scattering probe in the determination of HSA. Under optimum conditions, the response is linearly proportional to the concentration of HSA between 0.005 and 1.5 mg/L, and the detection limit is 3.5 μg/L. The proposed method has been applied to the analysis of albumin in human serum samples, and the recoveries were from 98% to 100.2%. |
| |
| Keywords: | TiO2 collosol Double scattering Human serum albumin |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
