多壁碳纳米管-分子印迹传感器测定盐酸克伦特罗

结合碳纳米材料和分子印迹技术,建立了以K3Fe(CN)6]为探针测定盐酸克伦特罗的方法。以邻苯二胺为功能单体,盐酸克伦特罗为模板,采用电化学聚合法在多壁碳纳米管修饰电极表面制备了分子印迹薄膜。用乙腈水溶液可快速去除模板,得到多壁碳纳米管-分子印迹传感器。用循环伏安法、交流阻抗法和石英晶体微天平技术对印迹膜进行了表征,膜厚为12.3 nm。K3Fe(CN)6]的相对峰电流与盐酸克伦特罗的浓度在4.0×10-8～6.6×10-6 mol/L范围内呈线性关系,检测限为8.1×10-9 mol/L。选择性实验表明传感器对结构类似物具有较强的抗干扰能力。此传感器可用于猪肉中盐酸克伦特罗的测定,加标回收率为101.3%～107.9%。

Determination of clenbuterol with multi-walled carbon nanotubes-molecular imprinted sensors

Based on the incorporation of multi-walled carbon nanotubes(MWNTs) and molecular imprinting technique,an electrochemical sensor for the determination of clenbuterol was developed.The sensor was prepared by electropolymerizing o-phenylenediamine(o-PD) on the MWNTs modified glassy carbon electrode in the presence of the template clenbuterol.The template can be quickly removed in acetonitrile solution.Cyclic voltammetry(CV),AC impedance(IMP) and quartz crystal microbalance technique(QCM) were employed to characterize the performance of the modified electrode,the thickness of the membrance was determined to be 12.3 nm.The sensor provided an efficient way to eliminate interferences from the compounds with similar structures.A linear response between the relative peak current change of potassium hexacyanoferrate(III) and the concentration of clenbuterol was obtained in the range of 4.0×10-8～6.6×10-6 mol/L and the detection limit was 8.1×10-9 mol/L based on the 3σ of blank signals.The sensor was successfully applied to the determination of clenbuterol in pork with the recoveries from 101.3% to 107.9%.