| 茜素红与蛋白质分子Langmuir吸附聚集反应研究 |
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| 引用本文： | 刘保生,孙艳梅,高静,刘智超,李雅冰.茜素红与蛋白质分子Langmuir吸附聚集反应研究[J].分析试验室,2005,24(8):67-69. |
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| 作者姓名： | 刘保生 孙艳梅 高静 刘智超 李雅冰 |
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| 作者单位： | 1. 河北大学理化分析中心,河北省分析科学技术重点实验室,保定,071002
2. 河北大学理化分析中心,河北省分析科学技术重点实验室,保定,071002;邯郸学院化学系,邯郸,056004 |
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| 基金项目： | 河北省教育厅博士科研项目 |
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| 摘 要： | 在pH4.3的B-R缓冲体系中,用微相吸附-光谱修正技术1]研究了茜素红(ARS)与牛血清白蛋白(BSA)、人血清白蛋白(HSA)的结合反应。其吸附结合常数分别为:KBSA-ARS=3.950×104,KHSA-ARS=4.377×104。染料与蛋白的最大结合数分别为NARS∶NBSA=9∶1,NARS∶NHSA=7∶1。经光谱修正技术计算结合产物的实际摩尔吸光系数分别为εBSA-ARS(537nm)=2.517×104L.mol-1.cm-1,εHSA-ARS(519nm)=2.051×104L.mol-1.cm-1,检出限BSA为19mg/L,HSA为23mg/L。经探讨该结合反应机理符合Langmuir吸附聚集反应方程。
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| 关 键 词： | 微相吸附-光谱修正技术 茜素红 血清白蛋白 Langmuir吸附聚集反应 |
| 文章编号： | 1000-0720（2005）08-0067-03 |
| 收稿时间： | 2004-09-08 |
| 修稿时间： | 2004-09-082004-11-17 |
Langmuir aggregation of alizarin red in protein macromolecule |
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| LIU Bao-sheng,SUN Yan-mei,GAO Jing,LIU Zhi-chao,LI Ya-bing.Langmuir aggregation of alizarin red in protein macromolecule[J].Chinese Journal of Analysis Laboratory,2005,24(8):67-69. |
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| Authors: | LIU Bao-sheng SUN Yan-mei GAO Jing LIU Zhi-chao LI Ya-bing |
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| Abstract: | The microphase adsorption-spectral correction (MPASC) technique is described and applied to the study on the interaction of Alizarin red (ARS) with two proteins: bovine serum albumin (BSA) and human serum albumin (HSA) at pH 4.3 Britton-Robinson buffer solution. The results show that the adsorption ratios of ARS to proteins are N_(ARS)N_(BSA)=91, N_(ARS)N_(HAS)=71, the adsorption constants are K_(ARS-BSA)=3.34610~4, K_(ARS-HAS)=4.48310~4 respectively and the real molar absorptivities are measured to be _(BSA-ARS)(537 nm)=2.51710~4 L/(molcm), _(HSA-ARS)(519 nm)=2.05110~4 L/(molcm). The detection limits are 19 mg/L for BSA, and 23 mg/L for HSA. The aggregation of ARS in protein has been applied to the analysis of samples with satisfactory results. |
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| Keywords: | Microphase adsorption-spectral correction technique Alizarin red Serum albumin Langmuir aggregation |
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