| 液相微萃取-高效液相色谱法分析葡萄汁中多酚类化合物 |
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| 引用本文： | 胡玉玲,常蓓蓓,罗学军,李攻科.液相微萃取-高效液相色谱法分析葡萄汁中多酚类化合物[J].分析试验室,2009,28(8). |
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| 作者姓名： | 胡玉玲 常蓓蓓 罗学军 李攻科 |
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| 作者单位： | 中山大学化学与化学工程学院,广州,510275 |
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| 基金项目： | 国家自然科学基金，广东省自然科学基金 |
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| 摘 要： | 建立了一种基于液相微萃取与高效液相色谱联用技术测定葡萄汁中鞣花酸、白藜芦醇和槲皮素的分析方法. 比较了单液滴液相微萃取和中空纤维液相微萃取两种萃取模式, 选择了单液滴液相微萃取作为3种多酚类化合物的液相微萃取模式. 考察了搅拌速度、萃取时间、料液相pH和料液相离子强度的影响. 鞣花酸、白藜芦醇和槲皮素的富集倍数分别为48.4、 79.4和155.8, 方法的线性范围为0.0050～5.0 μg/mL, 鞣花酸、白藜芦醇和槲皮素的检出限分别为0.015, 0.0020, 0.0080 μg/mL, 相对标准偏差分别为2.0%, 1.8%和1.7%. 用于实际样品葡萄汁的分析, 加标回收率在81.9%～102.3%之间.
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| 关 键 词： | 液相微萃取 高效液相色谱 葡萄汁 多酚 |
Determination of polyphenols in grape juice by liquid-phase microextraction coupled with high performance liquid chromatography |
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| HU Yu-ling,CHANG Bei-bei,LUO Xue-jun,LI Gong-ke.Determination of polyphenols in grape juice by liquid-phase microextraction coupled with high performance liquid chromatography[J].Chinese Journal of Analysis Laboratory,2009,28(8). |
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| Authors: | HU Yu-ling CHANG Bei-bei LUO Xue-jun LI Gong-ke |
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| Institution: | School of Chemistry and Chemical Engineering;Sun Yat-sen University;Guangzhou 510275 |
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| Abstract: | A novel method for the determination of ellagic acid,resveratrol and quercetin in grape juice by liquid-phase microextraction(LPME) coupled with high performance liquid chromatography(HPLC) was developed.The results of two different modes of LPME were compared,and the single drop microextraction exhibited better extraction efficiency.Effects of stirring speed,extraction time,ionic strength,and pH were investigated.The enrichment factors of ellagic acid,resveratrol and quercetin were 48.4,79.4 and 155.8,resp... |
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| Keywords: | Liquid-phase microextraction High performance liquid chromatography Grape Juice Polyphenols |
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