| 在线自动化柱前衍生-高效液相色谱法测定食品中组胺的研究 |
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| 引用本文： | 房科腾,谢东华,丁斌,贾东智,殷居易,崔晓美,倪梅林,彭锦峰.在线自动化柱前衍生-高效液相色谱法测定食品中组胺的研究[J].分析试验室,2008,27(12). |
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| 作者姓名： | 房科腾 谢东华 丁斌 贾东智 殷居易 崔晓美 倪梅林 彭锦峰 |
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| 作者单位： | 1. 宁波出入境检验检疫局,宁波,315012
2. 胜利油田胜利工程建设有限责任公司,东营,257000 |
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| 基金项目： | 浙江省分析测试科技计划 |
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| 摘 要： | 建立了在线自动化柱前衍生.高效液相色谱法测定食品中组胺的新方法.通过对测定过程中各个影响因素进行优化,如自动化衍生程序的设定,衍生试剂的用量,衍生体系pH影响等,确立了适宜的测定条件.在该条件下,对于组胺的检出限为0.01 μg/mL,在0.05～100 λg/mL范围内,线性关系良好(r2＞0.999).通过对样品基质进行加标,检出限为0.20 mg/kg.将所建立的方法应用于金枪鱼罐头,烟熏鲣鱼,冻鲭鱼等样品中组胺的测定,测得的组胺含量为0.59～167 mg/kg,加标回收率均大于97%,测定值的相对标准偏差均小于5%.所建立的方法适用于大量样品的常规分析测定.
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| 关 键 词： | 在线自动化柱前衍生 高效液相色谱法 组胺 食品 |
Study on determination of histamine in food by on-line automated pre-column derivatization coupled with HPLC |
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| FANG Ke-teng,XIE Dong-hua,DING Bin,JIA Dong-zhi,YIN Ju-yi,CUI Xiao-mei,NI Mei-lin,PENG Jin-feng.Study on determination of histamine in food by on-line automated pre-column derivatization coupled with HPLC[J].Chinese Journal of Analysis Laboratory,2008,27(12). |
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| Authors: | FANG Ke-teng XIE Dong-hua DING Bin JIA Dong-zhi YIN Ju-yi CUI Xiao-mei NI Mei-lin PENG Jin-feng |
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| Institution: | FANG Ke-teng1,XIE Dong-hua1,DING Bin1,JIA Dong-zhi2,YIN Ju-yi1,CUI Xiao-mei1,NI Mei-lin1 , PENG Jin-feng1 (1.Ningbo Entry-Exit Inspection , Quarantine Bureau,Ningbo 315012,2.Shengli Oil Field Shengli Engineering , Construction Co.Ltd.,Dongying 257000) |
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| Abstract: | This study proposed a new analytical method for the determination of histamine in food by on-line automated pre-column derivatization coupled with high performance liquid chromatography(HPLC).By optimizing the related parameters in determination such as the automated pre-column derivatization program,the amount of derivatization reagent,pH of the reaction system,the appropriate conditions for HPLC determination were set up.Under the established conditions,the limit of detection for histamine standard is 0.0... |
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| Keywords: | On-line automated pre-column derivatization HPLC Histamine Food |
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