| 银纳米簇荧光探针检测L-半胱氨酸 |
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| 引用本文： | 冯建军,张国,柴瑞涛,朱维晃,康倩文.银纳米簇荧光探针检测L-半胱氨酸[J].分析试验室,2020(1):48-52. |
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| 作者姓名： | 冯建军 张国 柴瑞涛 朱维晃 康倩文 |
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| 作者单位： | 西安建筑科技大学环境与市政工程学院;西安建筑科技大学化学与化工学院 |
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| 基金项目： | 国家自然科学基金(21205092);陕西省自然科学基金(2018JQ2053);西安建筑科技大学自然科学专项基金(ZR18039);西安建筑科技大学人才基金(RC1306)资助 |
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| 摘 要： | 常温下合成了二氢硫辛酸(DHLA)包裹的银纳米簇(AgNCs),并基于L-半胱氨酸对AgNCs的荧光猝灭现象构建了AgNCs荧光探针对Cys的检测方法。结果表明,在优化条件下,AgNCs的荧光猝灭程度和Cys浓度在2.0~100μmol/L范围内呈现良好的线性关系(R2=0.998),检出限为1.77μmol/L(S/N=3)。在人体血清样品中Cys检测的加标回收率为94.0%~102.4%。
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| 关 键 词： | L-半胱氨酸 二氢硫辛酸(DHLA) 银纳米簇(AgNCs) 荧光探针 荧光猝灭 |
Silver-nanocluster fluorescent probes for detection of L-cysteine |
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| FENG Jianjun,ZHANG Guo,CHAI Ruitao,ZHU Weiliuang,KANG Qianwen.Silver-nanocluster fluorescent probes for detection of L-cysteine[J].Chinese Journal of Analysis Laboratory,2020(1):48-52. |
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| Authors: | FENG Jianjun ZHANG Guo CHAI Ruitao ZHU Weiliuang KANG Qianwen |
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| Institution: | (School of Environmental and Municipal Engineering,Xi'an University of Architecture and Technology,Xi'an 710055;School of Chemistry and Chemical Engineering,Xi'an University of Architecture and Technology,Xi'an 710055) |
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| Abstract: | Dihydrolipoic acid(DHLA)capped silver nanoclusters(AgNCs)was prepared at room temperature.Based on the fluorescence quenching phenomenon of L-cysteine to AgNCs,a method for detection of L-cysteine by AgNCs fluorescent probe was proposed.The results showed that under the optimized conditions,the fluorescence quenching degree of AgNCs and L-cysteine concentration presented a good linear relationship(R2=0.998)with L-cysteine concentration in the range of 2.0-100μmol/L,and the detection limit was1.77μmol/L(S/N=3).Moreover,the probes were successfully applied in the detection of L-cysteine in samples of human serum with the average recoveries in the range of 94.0%-102.4%. |
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| Keywords: | L-cysteine dihydrolipoic acid silver nanoclusters fluorescent probes fluorescence quenching |
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