| 高效毛细管电泳紫外检测法分离检测射干苷和鸢尾黄素 |
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| 引用本文： | 陈盛余,邓光辉,张桂华,陈凯虹,廖慧.高效毛细管电泳紫外检测法分离检测射干苷和鸢尾黄素[J].分析试验室,2011,30(3):80-82. |
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| 作者姓名： | 陈盛余 邓光辉 张桂华 陈凯虹 廖慧 |
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| 作者单位： | 广西民族大学化学与生态工程学院,南宁,530006 |
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| 摘 要： | 建立了毛细管电泳法测定射干药材中的射干苷和鸢尾黄素.探讨了检测波长、缓冲体系、缓冲液浓度和pH、分离电压等对分离的影响.在最优条件为20 mmol/L硼砂(pH 9.0),分离电压20 kV,被测物在10 min内实现基线分离.射干苷和鸢尾黄素的回收率分别为94.4%、93.5%,RSD分别为3.3%、2.8%.该法已...
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| 关 键 词： | 毛细管电泳 射干 射干苷 鸢尾黄素 |
Seperation and determination of tectoridin and tectorigenin by capillary electrophoresis with UV detection |
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| CHEN Sheng-yu,DENG Guang-hui,ZHANG Gui-hua,CHEN Kai-hong,LIAO Hui.Seperation and determination of tectoridin and tectorigenin by capillary electrophoresis with UV detection[J].Chinese Journal of Analysis Laboratory,2011,30(3):80-82. |
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| Authors: | CHEN Sheng-yu DENG Guang-hui ZHANG Gui-hua CHEN Kai-hong LIAO Hui |
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| Institution: | CHEN Sheng-yu,DENG Guang-hui,ZHANG Gui-hua,CHEN Kai-hong and LIAO Hui(Ecological Project Institute,Guangxi University for Nationalities,Nanning 530006) |
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| Abstract: | A method for determination of tectoridin and tectorigenin in Rhizoma Belamcandae by capillary electrophoresis was developed.The effects of some important factors such as detection wavelength,buffer system,concentration and pH,separation voltage were investigated to acquire the optimum condition.The two analytes could be separated within 10 min at the separation voltage of 20 kV in a 20 mmol/L borate buffer(pH 9.0).The recoveries of tectoridin and tectorigenin were 94.4% and 93.5% and RSD were 3.3% and 2.8% ... |
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| Keywords: | Capillary electrophoresis Rhizoma belamcandae Tectoridin Tectorigenin |
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