| 海藻酸钠水凝胶固载细胞色素c的蛋白膜伏安法研究 |
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| 引用本文： | 张会娜,盖盼盼,段静,王钦赏,陈晔,王邃,郭智勇.海藻酸钠水凝胶固载细胞色素c的蛋白膜伏安法研究[J].分析试验室,2010,29(3). |
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| 作者姓名： | 张会娜 盖盼盼 段静 王钦赏 陈晔 王邃 郭智勇 |
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| 作者单位： | 宁波大学材料科学与化学工程学院,宁波市新型功能材料及其制备科学实验室-省部共建国家重点实验室培育基地,宁波,315211 |
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| 基金项目： | 宁波市自然科学基金(2006A610044);;宁波大学学科(xkl09071)项目资助 |
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| 摘 要： | 用海藻酸钠(SA)水凝胶将细胞色素c(cyt-c)固定在棱面热解石墨电极(EPPGE)表面,形成稳定的SA-cyt-c/EPPGE电极,采用蛋白膜伏安法(PFV)研究了cyt-c与电极之间的直接电化学和离子强度、pH、外加金属离子对其电化学行为的影响及其电催化性能。结果表明:cyt-c中血红素辅基Fe(Ⅲ)/Fe(Ⅱ)电对表现出准可逆行为,pH7.0、NaCl浓度为0.1 mol/L时电化学活性最高,外加金属离子则有抑制作用,SA-cyt-c/EPPGE可催化还原O2,有可能发展成为一种溶解氧的生物传感器。
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| 关 键 词： | 细胞色素c 海藻酸钠 蛋白膜伏安法 棱面热解石墨电极 直接电化学 |
Research on cytochrome c entrapped in sodium alginate hydrogel films by protein film voltammetry |
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| ZHANG Hui-na,GAI Pan-pan,DUAN Jing,WANG Qin-shang,CHEN Ye,WANG Sui,GUO Zhi-yong.Research on cytochrome c entrapped in sodium alginate hydrogel films by protein film voltammetry[J].Chinese Journal of Analysis Laboratory,2010,29(3). |
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| Authors: | ZHANG Hui-na GAI Pan-pan DUAN Jing WANG Qin-shang CHEN Ye WANG Sui GUO Zhi-yong |
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| Institution: | Faculty of Materials Science and Chemical Engineering;The State Key Laboratory Base of Novel Functional Materials and Preparation Science;Ningbo University;Ningbo 315211 |
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| Abstract: | Cytochrome c (cyt-c) entrapped in sodium alginate (SA) hydrogel was electrostatically bound to the surface of edge plane pyrolytic graphite electrode (EPPGE), and then a stable SA-cyt-c/EPPGE was prepared. Direct electrochemistry of cyt-c at the electrode was monitored by protein film voltammetry (PFV). The effects of ionic strength, pH and exterior substances on the direct electrochemistry, and electrocatalytic properties of cyt-c were also studied. The electrochemical behavior of heme Fe(Ⅲ)/Fe(Ⅱ) in cyt-c is quasi-reversible, and its electrochemical performance is the best when pH is 7.0 and the concentration of supporting electrolyte NaCl is 0.1 mol/L, however, it is inhibited by exterior metal ions. Catalytic reduction of oxygen could be achieved at the SA-cyt-c/EPPGE, which means that it might be developed into a kind of biological sensor for dissolved oxygen. |
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| Keywords: | Cytochrome c Sodium alginate Protein film voltammetry Edge plane pyrolytic graphite electrode Direct electrochemistry |
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