| 花菁-脱氧核糖核酸的共振光散射光谱及其分析应用 |
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| 引用本文： | 方芳,张志涛,朱昌青.花菁-脱氧核糖核酸的共振光散射光谱及其分析应用[J].分析试验室,2012(3):51-54. |
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| 作者姓名： | 方芳 张志涛 朱昌青 |
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| 作者单位： | 蚌埠医学院药学系;安徽师范大学化学与材料科学学院 |
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| 基金项目： | 安徽省教育厅自然科学基金项目(KJ2010B412)资助 |
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| 摘 要： | 研究了一种苯并噻唑阳离子花菁与脱氧核糖核酸(DNA)作用的共振光散射光谱,在pH 6.0的六次甲基四胺-HCl缓冲介质中,痕量DNA的加入使花菁在590nm的共振光散射强度显著增强。在最佳实验条件下,增强的共振光散射强度与DNA浓度具有良好的线性关系,据此建立了一种测定DNA的共振光散射光谱法。方法的线性范围为:小牛胸腺DNA(CT DNA),0～20μg/mL,鱼精子DNA(FS DNA),0～15μg/mL;检出限分别为0.005μg/mL和0.008μg/mL。该方法已用于合成样品中DNA的测定。
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| 关 键 词： | 花菁 共振光散射法 脱氧核糖核酸 |
Resonance light scattering spectral study of the interaction of cyanine dye with nucleic acids and its analytical applications |
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| FANG Fang,ZHANG Zhi-Tao,and ZHU Chang-Qing.Resonance light scattering spectral study of the interaction of cyanine dye with nucleic acids and its analytical applications[J].Chinese Journal of Analysis Laboratory,2012(3):51-54. |
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| Authors: | FANG Fang ZHANG Zhi-Tao and ZHU Chang-Qing |
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| Institution: | 1.Department of Pharmacy,Bengbu Medicine College,Bengbu 233030;2.College of Chemistry and Materials Science,Anhui Normal University,Wuhu 241000) |
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| Abstract: | The resonance light scattering(RLS) spectra of benzothiazole cationic cyanine with deoxyribonucleic acid(DNA) have been studied.The RLS intensity of cyanine at 590nm is greatly enhanced by the addition of trace amounts of DNA in pH 6.0 hexamethylene tetramine-HCl media.Under the optical experimental conditions,the enhanced RLS intensity was proportional to the concentration of DNA.The RLS method for the determination of DNA was established.The linear ranges of the method are 0-20μg/mL for CT DNA and 0-15μg/mL for FS DNA,and the detection limits are 0.005μg/mL and 0.008μg/mL respectively.The method has been applied to the determination of DNA in synthetic samples. |
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| Keywords: | Cyanine Resonance light scattering method Deoxyribonucleic acid |
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