| 过氧化氢-巯嘌呤氧化还原反应的研究及荧光分析应用 |
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| 引用本文： | 黄春保,潘祖亭.过氧化氢-巯嘌呤氧化还原反应的研究及荧光分析应用[J].分析试验室,2001,20(1):58-60. |
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| 作者姓名： | 黄春保 潘祖亭 |
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| 作者单位： | 1. 黄冈师范学院化学系,
2. 武汉大学化学系, |
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| 摘 要： | 研究了过氧化氢氧化巯嘌呤的反应条件及产物的荧光特性 ,提出了高灵敏荧光光度法测定巯嘌呤含量的新方法。在碱性介质和沸水浴加热条件下 ,过氧化氢能将巯嘌呤定量转化为 6-嘌呤磺酸钠 ,其体系的荧光强度与巯嘌呤浓度在6.0× 1 0 -8～ 1 .0× 1 0 -5mol/L范围内呈线性关系 ,检出限为 2 .4× 1 0 -8mol/L。该法已用于片剂中的巯嘌呤含量的测定。
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| 关 键 词： | 荧光光度法 氧化还原反应 巯嘌呤 药物分析 巯嘌呤 抗肿瘤药物 |
| 文章编号： | 1000-0720(2001)01-0058-03 |
Study on Redox Reaction of 6-Mercaptopurine with Hydrogen Peroxide and Its Analytical Application |
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| HUANG Chun-bao,Pan Zu-ting.Study on Redox Reaction of 6-Mercaptopurine with Hydrogen Peroxide and Its Analytical Application[J].Chinese Journal of Analysis Laboratory,2001,20(1):58-60. |
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| Authors: | HUANG Chun-bao Pan Zu-ting |
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| Abstract: | A new spectrofluorimetric method for determination of 6-mercaptopurine(6-MP) based on redox reaction of 6-MP with H2O2 in NaOH medium and boiling water bath was established. Under the optimum conditions, a linear ralationship between fluorescence intensity and concentration of 6-MP was found to be 6.0×10-8～1.0×10-5 mol.L-1,with detection limit of 2.4×10-8 mol.L-1. The method was applied to determine 6-MP in tablets with satisfactory results. |
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| Keywords: | Spectrofluorimetry Redox reaction 6-Mercaptopurine Pharmaceutical analysis |
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