| 2,4-二硝基氯苯衍生高效液相色谱法测定饮品中牛磺酸含量 |
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| 引用本文： | 张晓凤,邹晓莉,曾红燕.2,4-二硝基氯苯衍生高效液相色谱法测定饮品中牛磺酸含量[J].分析试验室,2012(6):46-49. |
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| 作者姓名： | 张晓凤 邹晓莉 曾红燕 |
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| 作者单位： | 四川大学华西公共卫生学院卫生检验教研室 |
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| 基金项目： | 科技部国家科技支撑计划课题项目(2006BAD27B02)资助助 |
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| 摘 要： | 建立了饮品中牛磺酸的高效液相色谱(HPLC)测定方法。采用2,4-二硝基氯苯90℃下衍生30 min,冷至室温后离心,取上清液10μL进样分析。以甲醇-KH2PO4为流动相梯度洗脱,360nm紫外检测,柱温20℃,流速1 mL/min。结果显示牛磺酸至少在0.02～200 mg/L范围内线性良好(r=0.999),回收率在97.5%～114.0%之间,保留时间和峰面积的相对标准偏差(RSD)(n=6)分别为0.40%和5.8%,方法已用于饮品中牛磺酸含量的测定。
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| 关 键 词： | 牛磺酸 高效液相色谱法 2 4-二硝基氯苯 饮品 |
Determination of taurine in drinks by high performance liquid chromatography with 2,4-dinitrochlorobenzene precolumn derivatization |
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| ZHANG Xiao-feng,ZOU Xiao-li and ZENG Hong-yan.Determination of taurine in drinks by high performance liquid chromatography with 2,4-dinitrochlorobenzene precolumn derivatization[J].Chinese Journal of Analysis Laboratory,2012(6):46-49. |
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| Authors: | ZHANG Xiao-feng ZOU Xiao-li and ZENG Hong-yan |
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| Institution: | (West China School of Public Health,Sichuan University,Chengdu 610041) |
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| Abstract: | A method of reversed phase high performance liquid chromatography(RP-HPLC) coupled with an ultraviolet detecor(UV) was developed for the determination of taurine in drinks.Samples were derivatized by 2,4-dinitrochlorobenzene for 30 minutes at 90 ℃ and then chromatographically separated on a C18 column.The binary mobile phase,which was composed of methanol and 0.01 mol/l monopotassium phosphate,was run at a flow rate of 1 mL/min flow rate and column temperature of 20 ℃.The recoveries were in the rang of 97.5%~114.0%.The linear range was 0.02~200 mg/L(r=0.999).The intra-and inter-day relative standard deviations(RSDs) were 0.40% and 5.8% respectively. |
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| Keywords: | Turine RP-HPLC 2 4-Dinitrochlorobenzene precolumn derivatization Drinks |
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