| 荧光探针法研究色氨酸二肽与DNA的相互作用 |
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| 引用本文： | 卢奎,冯业振,赵东欣,任海平,赵玉芬.荧光探针法研究色氨酸二肽与DNA的相互作用[J].分析试验室,2009,28(1). |
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| 作者姓名： | 卢奎 冯业振 赵东欣 任海平 赵玉芬 |
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| 作者单位： | 1. 河南工业大学化学化工学院,郑州,450001
2. 清华大学化学系,生命有机磷化学及化学生物学教育部重点实验室,北京,100084 |
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| 基金项目： | 国家自然科学基金，河南省杰出青年科学基金 |
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| 摘 要： | 以溴化乙锭(EB)为荧光探针,对色氨酸二肽(Trp-Trp)与小牛胸腺DNA的作用机制进行了研究,考查了酸度、离子强度及磷酸根离子对Trp-rrp与DNA相互作用的影响;结果表明,在ph 7.2的模拟体液条件下,Tro-Trp可与DNA发生相互作用;向DNA-EB体系中加入Trp-Trp时荧光逐渐减弱,这说明Trp-Trp 能与DNA发生作用.通过ScatehaId方程进一步分析发现,Trp-Trp对DNA-EB的影响表现为混合模式:一方面,Trp-Trp能中和DNA上的磷氧负离子,导致DNA链收缩,从而影响DNA的构象,使嵌入的EB从DNA中部分游离出来,荧光减弱;另一方面,Trp-Trp与EB也存在静电位点竞争.
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| 关 键 词： | 荧光光谱 色氨酸二肽 小牛胸腺DNA 相互作用 |
Study on the interaction of trp-trp and DNA by fluorescence probe |
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| LU Kui,FENG Ye-zhen,ZHAO Dong-xin,REN Hai-ping,ZHAO Yu-fen.Study on the interaction of trp-trp and DNA by fluorescence probe[J].Chinese Journal of Analysis Laboratory,2009,28(1). |
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| Authors: | LU Kui FENG Ye-zhen ZHAO Dong-xin REN Hai-ping ZHAO Yu-fen |
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| Institution: | 1.School of Chemistry and Chemical Engineering;Henan University of technology;Zhengzhou 450001;2.Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology of Ministry of Education;Department of Chemistry;Tsinghua University;Beijing 100084 |
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| Abstract: | The interaction between tryptophan dipeptide (Trp-Trp) and ct-DNA was studied by fluorescence spectros- copy using ethidium bromide (EB) as fluorescence probe,the effect factors such as acidity,salt effect and phosphoric acid ion on the interaction are investigated;It is showed that in Tris-HCl (pH 7.2),Trp-Trp can react with DNA. The fluorescence intensity of DNA-EB quenched with the increase of Trp-Trp.According to the equation of scatchard, influence of Trp-Trp on interaction of DNA-EB belongs to mixed m... |
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| Keywords: | Fluorescence spectrum Tryptophan dipeptide Calf Thymus DNA Interaction |
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