| 超高效液相串联质谱法同时测定中成药中5-甲氧基补骨脂素、8-甲氧基补骨脂素和欧前胡素 |
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| 引用本文： | 席海为,马强,王超,白桦,李强,雷海民.超高效液相串联质谱法同时测定中成药中5-甲氧基补骨脂素、8-甲氧基补骨脂素和欧前胡素[J].分析试验室,2010,29(12). |
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| 作者姓名： | 席海为 马强 王超 白桦 李强 雷海民 |
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| 作者单位： | 1. 北京中医药大学中药学院,北京,100102
2. 中国检验检疫科学研究院,北京,100123 |
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| 摘 要： | 建立了同时测定中成药都梁滴丸中5-甲氧基补骨脂素、8-甲氧基补骨脂素和欧前胡素的超高效液相-串联质谱检测方法。都梁滴丸以甲醇超声提取45 min,提取液稀释后经Waters Oasis HLB固相萃取小柱净化,采用WatersACQUITY UPLC BEHC18(50 mm×2.1 mm,1.7μm)色谱柱,以乙腈(A)和水(B)为流动相进行梯度洗脱。采用电喷雾电离正离子模式,多反应监测模式检测,以保留时间和离子比定性,外标法定量。方法的定量限8-甲氧基补骨脂素和5-甲氧基补骨脂素均为0.3 mg/kg;欧前胡素为0.75 mg/kg。3种化合物在25~500μg/L范围内均呈线性,相关系数r0.99。在高,中,低三种添加水平的平均回收率为82.0%~107.4%日内精密度(RSD%)为1.1%~12%。
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| 关 键 词： | 超高效液相色谱 串联质谱 固相萃取 都梁滴丸 甲氧基补骨脂素 |
Simultaneous determination of 5-methoxypsoralen,8-methoxypsoralen and imperatorin in duliang dropping pills by ultra performance liquid chromatography tandem mass spectrometry |
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| XI Hai-wei,MA Qiang,WANG Chao,BAI Hua,LI Qiang,LEI Hai-min.Simultaneous determination of 5-methoxypsoralen,8-methoxypsoralen and imperatorin in duliang dropping pills by ultra performance liquid chromatography tandem mass spectrometry[J].Chinese Journal of Analysis Laboratory,2010,29(12). |
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| Authors: | XI Hai-wei MA Qiang WANG Chao BAI Hua LI Qiang LEI Hai-min |
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| Institution: | XI Hai-wei1,MA Qiang2,WANG Chao2,BAI Hua2,LI Qiang1 and LEI Hai-min1(1.Beijing University of Chinese Medicine school of Chinese Pharmacy,Beijing 100102,2.Chinese Academy of Inspection and Quarantine,Beijing 100123) |
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| Abstract: | |
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| Keywords: | Ultra performance liquid chromatography Tandem mass spectrometry Solid Phase Extract Duliang Dropping Pills Methoxypsoralens Imperatorin |
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