| 用结晶紫为荧光指示剂筛选G-四链体的小分子配体 |
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| 引用本文： | 刘萍,陈凤英,张国春,任有良,刘启.用结晶紫为荧光指示剂筛选G-四链体的小分子配体[J].分析试验室,2012(4):4-7. |
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| 作者姓名： | 刘萍 陈凤英 张国春 任有良 刘启 |
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| 作者单位： | 陕西省商洛学院化学与化学工程系 |
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| 基金项目： | 商洛学院科研基金项目(11SKY011);陕西省教育厅科研基金项目(11jk0578)资助 |
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| 摘 要： | 基于结晶紫(CV)与G-四链体的特异性结合以及结晶紫和端粒DNA(G-DNA)、G-四链体作用后荧光强度的差异,以天然抗肿瘤中药槲皮素为研究对象,建立了一种简单、快速、无标记筛选G-四链体配体的方法。研究了槲皮素与G-DNA的相互作用,并考察了G-DNA在K+存在下形成G-四链体后与槲皮素的作用情况。该方法已用于筛选G-四链体的小分子配体。
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| 关 键 词： | G-四链体 槲皮素 结晶紫 端粒酶 小分子配体 |
Label-free selection of g-quadruplex-binding ligands using crystal violet as fluorescent indicator |
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| LIU Ping,CHEN Feng-ying,ZHANG Guo-chun,REN You-liang and LIU Qi.Label-free selection of g-quadruplex-binding ligands using crystal violet as fluorescent indicator[J].Chinese Journal of Analysis Laboratory,2012(4):4-7. |
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| Authors: | LIU Ping CHEN Feng-ying ZHANG Guo-chun REN You-liang and LIU Qi |
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| Institution: | (Department of Chemistry and Chemical Engineering,Shangluo University,Shangluo 726000) |
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| Abstract: | A simple,quick,and label-free method for the selection of G-quadruplex-binding ligands using quercetin as a research object was proposed,which was based on specific binding properties of crystal violet(CV) with G-quadruplex and the change of fluorescence intensities of crystal violet(CV) interacting with the G-DNA and G-quadruplex.This method has been used for selecting G-quadruplex-binding ligands. |
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| Keywords: | G-quadruplex Quercetin Crystal violet Telomerase Ligand |
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