高效液相色谱法检测六种氟喹诺酮类兽药残留前处理的优化

建立了一个检测动物源性食品中6种氟喹诺酮类药物残留的高效液相色谱方法。不同基质的样品前处理对检测影响较大:鱼、肉及肝脏等样品需经过乙腈-0.1 mol/L KH2PO4缓冲液提取,乙腈饱和的正己烷洗涤去除油脂;蛋及乳制品样品用正己烷饱和的乙腈提取,乙腈饱和的正己烷去脂。目标化合物采用高效液相色谱-荧光检测器检测,外标法定量。对市售鸡肉、猪肉、鸡蛋进行检测,添加10、20、50、100μg/kg浓度水平时,回收率在82%~105%之间,相对标准偏差在4%~12%之间,方法的检出限诺氟沙星、环丙沙星、沙拉沙星及单诺沙星为5.0μg/Kg,恩诺沙星、达氟沙星为3.0μg/Kg。

Optimization of pretreatmet for determination of six fluoroquinolones by liquid chromatography

A mothod for analyzing 6 fluoroquinolones(FQS) residues in animal derived foods by high performance liquid chromatography was proposed.The sample pretreatment of different substrates were optimized,fish,meat and liver samples were extracted by CH3CN-0.1 mol / L KH2PO4 buffer solution,eggs and dairy products extracted by CH3CN,then hexane was added to remove grease.Target compounds were detected by HPLC with a flurorescence detector,and quantified with external standard method.The recoveries of 6 FQS were 82...