| 基于脂质体信号增强癌胚抗原电化学免疫传感器的研究 |
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| 引用本文： | 杜璋璋,吴秀玲,马翠萍,马洪军.基于脂质体信号增强癌胚抗原电化学免疫传感器的研究[J].分析试验室,2012,31(2):17-21. |
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| 作者姓名： | 杜璋璋 吴秀玲 马翠萍 马洪军 |
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| 作者单位： | 1. 济宁职业技术学院生物与化学工程系,济宁,272037
2. 济宁医学院附属医院,济宁,272037 |
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| 基金项目： | 山东省教育厅科技计划项目 |
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| 摘 要： | 研究了在玻碳电极利用巯基乙胺固定纳米金、然后纳米金固载癌胚抗体(Ab1),采用脂质体包裹电子媒介体硫堇,脂质体周围联接标记辣根过氧化物酶(HRP)的癌胚抗体(Ab2)对其传感器进行信号放大,通过循环伏安法考察了该免疫传感器的电化学特性,在优化的实验条件下,该免疫传感器的峰电流随着检测溶液中癌胚抗原(CEA)浓度的增大而增大,并在0.05～200 ng/mL CEA范围内呈现线性关系,回归方程为:Δi=0.20+0.24ρ(ng/mL);检测限为:18pg/mL(R=0.9947)。该免疫传感器可用于临床上对CEA的检测。
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| 关 键 词： | 癌胚抗原 脂质体 电化学免疫传感器 |
Study on an electrochemical immunosensor based on lipsome signal enhancing detection of carcinoembryonic antigens |
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| DU Zhang-zhang
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WU Xiu-ling
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MA Cui-ping
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MA Hong-jun.Study on an electrochemical immunosensor based on lipsome signal enhancing detection of carcinoembryonic antigens[J].Chinese Journal of Analysis Laboratory,2012,31(2):17-21. |
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| Authors: | DU Zhang-zhang WU Xiu-ling MA Cui-ping MA Hong-jun |
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| Institution: | 1.Department of Biology and Chemical Engineering,Jining Vocational Technology College,Jining 272037;2.Affitiated Hospital of Jining Medical College,Jining 272037) |
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| Abstract: | A novel electrochemical immunosensor for the detection of CEA has been developed by immobilizing anti-CEA on a AuNPs modified glassy carbon electrode with AET.The signal was enhanced by lipsome-capped thionine and lipsome-linked Ab2.The modified electrode was characterized by cyclic voltammetry.Under the optimum conditions,the detection is based on the peak current change of the modified electrode before and after the antigen-antibody reaction.The linear range is 0.05～200 ng/mL.The linear regression equation is Δi= 0.20 + 0.24 ρ(ng/mL)(r=0.9947).The detection limit is 18 pg/mL.The sensor has good reproducibility,selectivity and stability,and has been applied to the determination of human serum with satisfactory result. |
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| Keywords: | Carcinoembryonic Antigens Lipsome Electrochemical Immunosensor |
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