柱后加碱脉冲积分安培检测-离子色谱法测定水产品中十种生物胺

建立了利用淋洗液自动发生梯度淋洗的离子交换色谱法同时测定水产品中酪胺、5-羟色胺、腐胺、尸胺、组胺、庚二胺、苯乙胺、亚精胺、精胺、色胺等10种生物胺的方法。样品经处理后用Ionpac CS17分离柱和Ionpac CG17型保护柱分离,以EG40自动淋洗液发生器生成的5.0～55 mmol/L的MSA为淋洗液梯度洗脱,脉冲积分安培检测器检测。对梯度进行优化,10种生物胺都能基线分离,并且浓度和峰面积在一定范围内呈良好的线性关系。检出限在0.04 mg/kg以下,回收率在91.2%～102.5%之间,样品的RSD(n=6)小于5%。方法可用于水产品的检测。

Simultaneous determination of biogenic amines in aquatic products by ion exchange chromatography coupled with IPAD

A method was developed for the simultaneous determination of tyramine、serotonin hydrochloride、1,4-diaminobutane、cadaverine dihydrochloride、histamine、1,7-diaminoheptane、phenylethylamine、spermidine、spermine and tryptamine in aquatic products by gradient elution/ion exchange chromatograph.5.0-55 mmol/L methylsulfonic acid(MSA) was produced by an EG40 eluent autogenerator to be used as an eluent.The separation was performed on IonPac CS17 column and Ionpac CG17 column,and detected-ion was done by pulsed integrated amperometric electrochemical detection(IPAD).The gradient elution conditions were optimized.Ten kinds of biogenic amines could be separated and showed good linear relationship between the mass concentration and the peak area in the measurement ranges.The detection limits were below 0.04 mg/kg,the average recoveries were 91.2～102.5% and the relative standard deviations(RSD,n=6) were below 5%.The method is simple and has been applied to the detection of ten kinds of biogenic amines in aquatic products samples with good precision and accuracy.The results are satisfactory,and this method can be used to evaluate if the aquatic products has been bad.