西番莲果中嘧霉胺残留的SPE-RP-HPLC降解分析

建立固相萃取-反相高效液相色谱法测定西番莲果中嘧霉胺的残留降解分析的方法.色谱柱为Shim-pack VP-ODS 150 mm×4.6 mm,流动相为甲醇:1％冰乙酸=90:10(V/V),流速为0.4 mL/min,进样量20μL.嘧霉胺在0.0760～21.20 mg/L(r=0.9964)范围内与峰面积呈良好线性关系,检出限为0.05 mg/L,嘧霉胺的回收率为85.6％～102.3％,相对标准偏差0.89％～2.7％.方法可作为西番莲果中嘧霉胺含量监测的控制方法.同时,还对嘧霉胺由西番莲果皮向西番莲果肉的迁移,在西番莲果体上的降解动态进行了测定.

Degradation analysis of pyrimethanil residue in passion fruit by RP-HPLC with solid-phase extraction

A reversed-phase high performance liquid chromatograghic method with solid-phase extraction was used for the degradation analysis of pyrimethanil residue in passion fruit.The HPLC conditions were as follows: the column was Shim-pack VP-ODS 150 mm×4.6 mm.CH3OH: 1% CH3COOH = 90:10(V/V)as mobile phase,flow rate 0.4 mL /min,injection volume 20 μL and detection wavelength at 270 nm.Good linearity of pyrimeth-anil was obtained over the range of 0.0760～21.20 mg/L(r=0.9964).The detection limit was 0.0500 mg/L for pyrimethanil.The average recoveries ranged from 85.6% to 102.3% with relative standard deviation of 0.89%～2.7%.The method is fast and simple for the residue detection and analysis of pyrimethanil in passion fruit.and it is suitable for the determination of fungicide residue of pyrimethanil in passion fruit.