| 微流控芯片电泳激光诱导荧光检测p53外显子上的突变点 |
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| 引用本文： | 李钢,葛淑丽,王清江,何品刚,方禹之.微流控芯片电泳激光诱导荧光检测p53外显子上的突变点[J].分析试验室,2010,29(12). |
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| 作者姓名： | 李钢 葛淑丽 王清江 何品刚 方禹之 |
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| 作者单位： | 华东师范大学化学系; |
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| 基金项目： | 教育部新世纪人才支持计划，上海市科委项目 |
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| 摘 要： | 利用单链构象多态性(SSCP),建立微流控芯片电泳(ME)联合激光诱导荧光检测(LIF)技术,检测人类p53基因点突变的方法。设计不同碱基长度的p53单链序列,针对易突变的外显子7,8,9进行SSCP分析,分离野生与突变的单链DNA序列;研究了筛分介质聚乙烯基氧化物(PEO)的浓度,场强对芯片电泳行为的影响。在PEO质量分数为0.5%,分离场强为260V/cm时,100 s之内就可以实现样品p53外显子7,8,9的野生型与突变型碱基的分离检测。
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| 关 键 词： | 微流控芯片电泳 激光诱导荧光检测 单链构象多态性 p53 |
p53 Gene mutation detection exon 7-9 by microchip electrophoresis with single strand conformation polymorphism |
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| LI Gang,GE Shu-li,WANG Qing-jiang,HE Pin-gang,FANG Yu-zhi.p53 Gene mutation detection exon 7-9 by microchip electrophoresis with single strand conformation polymorphism[J].Chinese Journal of Analysis Laboratory,2010,29(12). |
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| Authors: | LI Gang GE Shu-li WANG Qing-jiang HE Pin-gang FANG Yu-zhi |
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| Institution: | LI Gang,GE Shu-li,WANG Qing-jiang,HE Pin-gang and FANG Yu-zhi(Department of Chemistry,East China Normal University,Shanghai 200062) |
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| Abstract: | This study examined the potential of microchip electrophoresis with a laser-induced fluorescence detector using a conventional glass microchip for the fast detection and simultaneous analysis of mutations in the p53 gene of exon 7,8 and 9.The separation efficiency at various polyethyleneoxide concentrations and the injection electric field were investigated.The mutant and normal ssDNA in p53 gene were analyzed within 100 s under the optimized conditions.The microchip electrophoresis with a laser-induced flu... |
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| Keywords: | Microchip electrophoresis Laser-induced fluorescence detector SSCP p53 |
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