金纳米微粒作探针共振瑞利散射光谱法测定盐酸异丙嗪

在pH 1.8～3.0的酸性介质中,质子化的盐酸异丙嗪(PMZ)可与带负电荷的金纳米微粒依靠静电和疏水作用相互结合,导致共振瑞利散射(RRS)强度显著增强,其最大散射峰位于368 nm,并在284,440,498 nm处有明显的散射峰,在选定的测量波长下,盐酸异丙嗪在0.04～0.10μg/mL的浓度范围内与RRS强度成正比,该法具有高的灵敏度,其检出限为1.34 ng/mL。考察了体系的RRS光谱特征,研究了适宜的反应条件、影响因素,研究了共存物质的影响,据此建立了金纳米微粒作探针RRS法测定盐酸异丙嗪的新方法。

Resonance rayleigh scattering spectral method for determination of promethazine hydrochloride with gold nanoparticles as probe

In media of pH 1.8～3.0,gold nanoparticles with negative charge prepared by sodium citrate reduction method can react with protonated promethazine hydrochloride(PMZ) to form a bigger binding product by the electrostatic attraction and hydrophobic interaction.This resulted in a remarkable enhancement of RRS and the appearance of a new RRS spectrum.The maximum RRS peak was at 368 nm,and there are three smaller scattering peaks that were at 284 nm,440 nm and 498 nm,respectively.The enhanced RRS intensity((I) was directly proportional to the concentration of PMZ.The quantitative determination range and detection limit(3σ) of the system are 0.04～0.10 μg/mL and 1.34 ng/mL.The characteristic of RRS spectra,the optimum condition for the reaction and influencing factors were studied.The effect of coexistent substance on the determination of PMZ has been investigated.Gold nanoparticles could be the RRS probe for the determination of PMZ.