电化学衍生-高效液相色谱和荧光光度法检测花生酱中的黄曲霉毒素

建立了免疫亲和柱净化-柱后电化学衍生-高效液相色谱结合荧光光度法检测花生酱中4种黄曲霉毒素(B1、B2、G1和G2)的方法。样品经过体积分数为60%的甲醇提取,通过免疫亲和柱净化后,以KobraCell装置柱后衍生,高效液相色谱法分离定量。黄曲霉毒素B1、B2、G1和G2能达到完全的基线分离,检测限分别为0.5、0.15、0.5和0.15μg/kg,线性相关系数0.999,回收率可达74.2%～96.5%,相对标准偏差低于11%。该方法能够满足花生酱中黄曲霉毒素检测的需要。

Determination of aflatoxins in peanut butter by combining HPLC with electrochemical derivatization and fluorometry

A method was developed for the determination of aflatoxins (B_1, B_2,G_1 and G_2) in peanut butter by fluorometry and immunoaffinity column clean-up with high performance liquid chromatography (HPLC) using post-column electrochemical derivatization. Peanut butter samples were extracted with methanol-water(60∶40, V/V). The extract was purified on an immunoaffinity column and the toxins were separated and quantified by HPLC using post-column kobra cell derivatization. Baseline of aflatoxin B_1,B_2,G_1 and G_2 could be separated completely, the limits of detection were 0.5, 0.15, 0.5 and 0.15 μg/kg respectively. The linear relationship within the range was better with correlation coefficien t>0.999. Recoveries of aflatoxin B_1,B_2,G_1 and G_2 were between 74.2% and 96.5%, and the relative standard deviations were all below 10.7%. This method is convenient, rapid, sensitive and exact. It would meet the need of the determination of aflatoxins (B_1,B_2,G_1 and G_2) in peanut butter.