固相萃取-高效液相色谱法同时测定食品中4种合成色素

以可代替使用的水溶性合成色素-亮黑BN、固绿FCF、酸性红、酸性黄36为研究对象,建立了高效液相色谱同时测定食品中4种合成色素的方法。样品通过WAX固相萃取柱富集净化,10%氨化甲醇洗脱,采用C18色谱柱分离,以甲醇和10 mmol/L乙酸铵水溶液作为流动相梯度洗脱,外标法定量。通过固相萃取柱净化后,杂质对待测物的干扰明显降低,优化色谱条件下,4种色素在0.10~10.0 mg/L质量浓度范围内,线性良好,相关系数(r)≥0.9995。方法回收率为92.6%~101.8%,相对标准偏差(RSD)≤5.4%,方法检出限为0.01~0.04 mg/kg。该方法灵敏度高,准确可靠,适合食品中亮黑BN、固绿FCF、酸性红、酸性黄36的同时测定。

Simultaneous determination of four pigments in food by solid phase extraction combined with high performance liquid chromatography

An analytical method for the simultaneous determination of four pigments including brilliant black BN,fast green FCF,azorubine and acid yellow 36 in food was developed by solid phased extraction(SPE)-high performance liquid chromatography.The samples were purified and enriched with WAX cartridge with 10%ammoniated methanol as elution,then the analytes were separated by C18 column using a mobile phase consisting of methanol and 10 mmol/L ammonium acetate in gradient mode.Clean extraction was achieved using SPE operation,and the interferences were well excluded.Under optimizing chromatographic conditions,the four pigments showed good linearity in the range of 0.10~10.0 mg/L(r≥0.9995).The recoveries were92.6%-101.8%,the relative standard deviations(RSDs)were less than 5.4%,and the detection limits were0.01-0.04 mg/kg.The method was accurate and sensitive,which was suitable for the simultaneous detection of brilliant black BN,fast green FCF,azorubine and acid yellow 36 in food.