| 流动注射比浊法测定人体血清总蛋白 |
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| 引用本文： | 汪敬武,王兆喜,熊和玉.流动注射比浊法测定人体血清总蛋白[J].分析试验室,2004,23(8):78-81. |
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| 作者姓名： | 汪敬武 王兆喜 熊和玉 |
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| 作者单位： | 南昌大学化学系,南昌,330047;温州师范学院,温州,325027 |
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| 摘 要： | 基于磺基水杨酸 硫酸钠混合试剂与蛋白质发生浊度反应,设计了具有一个汇合点的FIA单道流路,运用正交实验优化了影响比浊反应的主要因素,并探讨了流动注射分析条件,从而提出了流动注射比浊法测定人血清中总蛋白的新方法。本法实验结果与双缩脲法一致,每小时进样60次,线性范围为0.14～1.40mg mL。
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| 关 键 词： | 流动注射比浊法 总蛋白 人体血清 |
| 文章编号： | 1000-0720(2004)08-0078-04 |
Determination of total proteins in human serum by flow-injection-turbidimetry |
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| WANG Jing-wu,XIONG He-yu.Determination of total proteins in human serum by flow-injection-turbidimetry[J].Chinese Journal of Analysis Laboratory,2004,23(8):78-81. |
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| Authors: | WANG Jing-wu XIONG He-yu |
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| Abstract: | Based on the turbidimetric interaction between proteins and sulfosalicylic acid-Na_2SO_4, a flow injection analysis (FIA) method for determination of total proteins in human serum was proposed. The optimum conditions of the determination were studied. Calibration curve of absorbance versus concentration was linear from 0.14 to 1.40 mg/mL with a correlation coefficient of 0.996. The average sampling rate was about 60 samples per hour. |
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| Keywords: | Flow-injection-turbidimetry Total proteins Human serum |
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