| 新型环氧水解酶手性拆分环氧氯丙烷的气相色谱法 |
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| 引用本文： | 卢定强,涂清波,凌岫泉,王俊,党安旺,李衍亮,陈佳,常亚军.新型环氧水解酶手性拆分环氧氯丙烷的气相色谱法[J].分析试验室,2010,29(4). |
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| 作者姓名： | 卢定强 涂清波 凌岫泉 王俊 党安旺 李衍亮 陈佳 常亚军 |
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| 作者单位： | 1. 南京工业大学药学院,南京,210009;江苏省药物研究所,南京,210009
2. 南京工业大学药学院,南京,210009 |
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| 基金项目： | 国家自然科学基金(20876076);;国家重点基础研究发展计划(2009CB724700)项目资助 |
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| 摘 要： | 建立了一种用于快速测定生物酶转化体系中手性环氧氯丙烷的气相色谱法。采用ZKAT-ChiralB毛细管柱,FID检测器检测,内标法计算含量。(R)-环氧氯丙烷和(S)-环氧氯丙烷在0.012～0.461g/mL呈良好的线性关系,RSD为1.3%、1.7%,回收率为97.6%、101.3%。
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| 关 键 词： | 气相色谱 酶转化 手性 环氧氯丙烷 |
Separation and determination of chiral epichlorohydrin by gas chromatography with a new epoxy hydrolase |
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| LU Ding-qiang,TU Qing-bo,LING Xiu-quan,WANG Jun,DANG An-wang,LI Yan-liang,CHEN Jia,CHANG Ya-jun.Separation and determination of chiral epichlorohydrin by gas chromatography with a new epoxy hydrolase[J].Chinese Journal of Analysis Laboratory,2010,29(4). |
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| Authors: | LU Ding-qiang TU Qing-bo LING Xiu-quan WANG Jun DANG An-wang LI Yan-liang CHEN Jia CHANG Ya-jun |
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| Institution: | 1.School of Pharmaceutical Sciences;Nanjing University of Technology;Nanjing 210009;2.Jiangsu Institute of Materia Medica;Nanjing 210009 |
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| Abstract: | A method for determining chiral epichlorohydrinin in the system of enzymatic transformation by gas chromatography was developed.The chiral epichlorohydrinin was analyzed by gas chromatography with ZKAT-Chirl B capillary column and flameionization detection using an internal standard method.(R)-epichlorohydrin and (S)-epichlorohydrin showed a good linear relationship in the range from 0.012 to 0.461 g/mL.The precision test show that the RSDs were 1.3% and 1.7%,and the recoveries were 97.6% and 101.3%. |
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| Keywords: | Gas chromatography Enzymatic transformation Chiral Epichlorohydrin |
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