| 高效液相色谱共振瑞利散射联用方法检测大鼠血清中的西索米星 |
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| 引用本文： | 甘杰,张丹,罗岳平,田耘,黄东勤.高效液相色谱共振瑞利散射联用方法检测大鼠血清中的西索米星[J].分析试验室,2011,30(9):100-104. |
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| 作者姓名： | 甘杰 张丹 罗岳平 田耘 黄东勤 |
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| 作者单位： | 1. 湖南省环境监测中心站,长沙,410014
2. 吉林省前郭县第一中学,松原,138000 |
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| 摘 要： | 用高效液相色谱与共振瑞利散射方法联用对西索米星进行检测.采用滂胺天蓝(PSB)作为分子识别探针,C18反相色谱柱进行分离.流动相为含0.22％三氟乙酸的20 mmol/L醋酸钠和甲醇(92:8,V/V),流速为0.5 mL/min.柱后探针溶液流速为0.1 mL/min.共振瑞利散射(RRS)检测波长设为λex=λem...
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| 关 键 词： | 高效液相色谱 共振锐利散射 西索米星 |
Determination of sisomicin in rat serum by high performance liquid chromatography coupled with resonance rayleigh scattering technique |
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| GAN Jie,ZHANG Dan,LUO Yue-ping,TIAN Yun,HUANG Dong-qin.Determination of sisomicin in rat serum by high performance liquid chromatography coupled with resonance rayleigh scattering technique[J].Chinese Journal of Analysis Laboratory,2011,30(9):100-104. |
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| Authors: | GAN Jie ZHANG Dan LUO Yue-ping TIAN Yun HUANG Dong-qin |
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| Institution: | GAN Jie 1,ZHANG Dan2,LUO Yue-ping1,TIAN Yun1 and HUANG Dong-qin1(1.Hunan Province Enviromental Monitoring Central Station,Changsha 410014,2.Jilin Province Qianguo County First Middle School,Songyuan 138000) |
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| Abstract: | A selective and rapid high-performance liquid chromatography with resonance Rayleigh scattering detection(HPLC-RRS) was developed for separation and determination Sisomicin,etimicin.Pontamine sky blue(PSB) was chosen as molecular recognition probe,which could forms ion-association complex with Sisomicin in mobile medium and produce strong scattering signal.The separation was performed on a C18 reversed phase column.The mobile phase consisted of 0.22 % trifluoroacetic acid 20 mmol/L sodium acetate solution a... |
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| Keywords: | High Performance Liquid Chromatography Resonance Rayleigh Scattering Sisomicin |
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