| Cu（Ⅱ）-H2O2-考马斯亮蓝G250体系催化光度法测定痕量铜 |
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| 引用本文： | 严进.Cu（Ⅱ）-H2O2-考马斯亮蓝G250体系催化光度法测定痕量铜[J].分析试验室,2012(8):83-85. |
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| 作者姓名： | 严进 |
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| 作者单位： | 南通职业大学化学与生物工程学院 |
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| 摘 要： | 在NH3-NH4Cl缓冲溶液介质中,痕量Cu2+对H2O2氧化考马斯亮蓝G250(CBBG)褪色反应的显著催化作用,建立了催化动力学光度法测定痕量铜的新方法。线性范围为0.02～0.20μg/mL,检出限为1.34×10-9g/mL。考察了20多种共存离子的影响。方法用于发样中痕量铜离子的测定,相对标准偏差小于3.4%,加标回收率为97%～105%。
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| 关 键 词： | 催化动力学 铜 过氧化氢 考马斯亮蓝G250 |
Study on catalytic spectrophotometric determination of trace copper with copper(Ⅱ)-hydrogen peroxide-coomassie brilliant blue G250 system |
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| YAN Jin.Study on catalytic spectrophotometric determination of trace copper with copper(Ⅱ)-hydrogen peroxide-coomassie brilliant blue G250 system[J].Chinese Journal of Analysis Laboratory,2012(8):83-85. |
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| Authors: | YAN Jin |
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| Institution: | YAN Jin(School of Chemical and Biological Engineering,Nantong Vocational College,Nantong 226007) |
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| Abstract: | Copper(Ⅱ) catalyze the oxidation of coomassie brilliant blue G250(CBBG)by hydrogen peroxide in NH3-NH4Cl buffer solution and copper(Ⅱ) can be determined by spectrophometry with the decolorization effete.Therefore,a new sensitive catalytic kinetics spectrophotometric method is proposed.The range of determination for copper(II) is 0.02～0.20μg/mL.The detection limit of the method is 1.34×10-9g/mL.The interferences of foreign ions are also studied.The procedure has been used to determine copper(Ⅱ) in human hair,with the relative standard deviation of below 3.4% and the average recovery of 97%～105%. |
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| Keywords: | Catalytic kinetic analysis Copper(Ⅱ) Hydrogen peroxide Coomassie brilliant blue G250 |
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