傅里叶变换近红外光谱法快速检测人血清生化成分

应用傅里叶变换近红外光谱透射技术结合偏最小二乘法(PLS)建立了人血清中7种生化成分的定标模型,利用内部交叉验证和自动优化功能对定标模型进行了优化,确定了最优建模参数。模型对人血清中总胆固醇、甘油三酯、总蛋白、白蛋白、载脂蛋白B、低密度脂蛋白胆固醇、葡萄糖定标样品集的预测值与化学值的相关系数r分别为0.9011、0.9593、0.9249、0.761、0.8831、0.5191、0 9148,预测校正标准误差RMSECV分别为15mg/dL,21.6mg/dL,2 66g/L,3 96g/L,0.091g/L,16.2mg/dL,0.49mmol/L。

Rapid determination of biochemical compounds in human serum using Fourier transform near infrared spectroscopy

Seven biochemical compounds in human serum were analyzed rapidly using Fourier transform near infrared transmittance spectroscopy(NIRS) combined with partial least square regrassion(PLS). The optimum parameters of mathematics models for them were studied using leave-one-out cross validation method. The correlation coefficients(r) between the true values and the NIRS prediction values for total cholesterol, glycerinate, total protein, albumin, apolipoprotein B, low-density lipoprotein(LDL) cholesterol and glucose of the calibration sample sets were 0.9011, 0.9593, 0.9249, 0.761, 0.8831, 0.5191, 0.9148 and the root mean square errors of cross validation (RMSECV) for them were 15.0 mg/dL, 21.6 mg/dL, 2.66 g/L, 3.96 g/L, 0.091 g/L, 16.2 mg/dL,0.49 mM/L respectively.