| 快速检测中药材中黄曲霉毒素B1的电化学生物传感器 |
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| 引用本文： | 魏小红,尤金坤,王彤颖,余宇燕,张红艳.快速检测中药材中黄曲霉毒素B1的电化学生物传感器[J].分析试验室,2021,40(2):130-134. |
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| 作者姓名： | 魏小红 尤金坤 王彤颖 余宇燕 张红艳 |
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| 作者单位： | 福建中医药大学药学院,福州350122;福建中医药大学药学院,福州350122;福建中医药大学药学院,福州350122;福建中医药大学药学院,福州350122;福建中医药大学药学院,福州350122 |
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| 基金项目： | 国家自然科学基金(81703695);福建省教育厅新世纪人才计划项目;福建中医药大学中药制剂与质量控制科研平台(X2019009-平台)项目;福建中医药大学校管(X2019008-重点)项目资助。 |
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| 摘 要： | 结合新型纳米传感膜材料,以黄曲霉毒素B1(AFB1)单克隆抗体为生物识别元件,通过考察AFB1与抗体之间的相互作用对测试底液中Fe(CN)6]3-/Fe(CN)6]4-(Fe(CN)6]3-/4-)氧化还原体系的影响,构建了一种可用于中药材中AFB1快速检测的电化学生物传感器。在最佳条件下,对AFB1浓度的线性响应范围为0.1~100 pmol/L,检出限为14.7 fmol/L。应用该方法测定大枣、薏苡仁、决明子中AFB1的含量,加标回收率在95.3%~109.5%之间。
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| 关 键 词： | 中药材 快速检测 黄曲霉毒素B1 电化学生物传感纳米传感膜材料 |
Electrochemical biosensor for rapid detection of AFB1 in Chinese herbal medicine |
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| WEI Xiaohong,YOU Jinkun,WANG Tongying,YU Yuyan,ZHANG Hongyan.Electrochemical biosensor for rapid detection of AFB1 in Chinese herbal medicine[J].Chinese Journal of Analysis Laboratory,2021,40(2):130-134. |
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| Authors: | WEI Xiaohong YOU Jinkun WANG Tongying YU Yuyan ZHANG Hongyan |
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| Institution: | (Pharmacy College of Fujian University of Traditional Chinese Medicine,Fuzhou 350122) |
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| Abstract: | An electrochemical biosensor with strong specificity,high sensitivity and wide linear range was established to detect aflatoxin Bl(AFB1)in Chinese herbal medicine.In this method,the anti-AFB1 monoclonal antibodies was employed as a recognition element,and the novel nano-sensing membrane material was used to immobilize antibodies.Meanwhile,the effect of the interaction between AFB1 and antibody on theFe(CN)6]3-/Fe(CN)6]4-(Fe(CN)6]3-/4-)redox system was studied by differential pulse voltammetry(DPV)to detect AFB1.Under optimal conditions,a linear response of AFB1 concentration was obtained over the range from 0.1 to 100 pmol/L with detection limit of 14.7 fmol/L.The proposed method was used to analyse samples of Chinese herbal medicine,the obtained values of the recovery were in the range of 95.3%-109.5%. |
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| Keywords: | Chinese herbal medicine rapid detection aflatoxin B1 nano-sensing membrane materials for electrochemical biosensor |
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